An improved method for utilizing high‐throughput amplicon sequencing to determine the diets of insectivorous animals

DNA analysis of predator faeces using high‐throughput amplicon sequencing (HTS) enhances our understanding of predator–prey interactions. However, conclusions drawn from this technique are constrained by biases that occur in multiple steps of the HTS workflow. To better characterize insectivorous an...

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Veröffentlicht in:	Molecular ecology resources Jg. 19; H. 1; S. 176 - 190
Hauptverfasser:	Jusino, Michelle A., Banik, Mark T., Palmer, Jonathan M., Wray, Amy K., Xiao, Lei, Pelton, Emma, Barber, Jesse R., Kawahara, Akito Y., Gratton, Claudio, Peery, M. Zachariah, Lindner, Daniel L.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	England Wiley Subscription Services, Inc 01.01.2019
Schlagworte:	AMPtk animal manures Aquatic insects arthropod mock community Arthropoda Arthropods bat guano Bats Bioinformatics Chiroptera Communities Community Culicidae cytochrome-c oxidase Cytochromes Deoxyribonucleic acid Diet dietary analysis DNA DNA sequencing Dung Economic conditions feces Guano insectivore insectivores Insects Mitochondria mitochondrial genes Mosquitoes next‐generation sequencing oligodeoxyribonucleotides Parameterization Pitfall traps Polymerase chain reaction Predator-prey interactions predator-prey relationships Prey Primers sequence analysis Workflow arthropod mock community AMPtk dietary analysis next-generation sequencing bat guano insectivore
ISSN:	1755-098X, 1755-0998, 1755-0998
Online-Zugang:	Volltext
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