Evaluation of rapid prenatal human immunodeficiency virus testing in rural cameroon

Pregnant women (n = 859) in rural Cameroonian prenatal clinics were screened by two rapid human immunodeficiency virus (HIV) antibody tests (rapid tests [RT]) (Determine and Hema-Strip) using either whole blood or plasma. One additional RT (Capillus, HIV-CHEK, or Sero-Card) was used to resolve disco...

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Veröffentlicht in:Clinical and diagnostic laboratory immunology Jg. 12; H. 7; S. 855
Hauptverfasser: Granade, Timothy C, Parekh, Bharat S, Tih, Pius M, Welty, Thomas, Welty, Edith, Bulterys, Marc, Ndikintum, George, Nkuoh, Godlove, Tancho, Samuel
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.07.2005
Schlagworte:
Algorithms
Cameroon
Female
HIV
HIV Antibodies - blood
HIV Infections - blood
HIV Infections - diagnosis
HIV Seropositivity - blood
Humans
Predictive Value of Tests
Pregnancy
Pregnancy Complications, Infectious - blood
Pregnancy Complications, Infectious - diagnosis
Cameroon
ISSN:1071-412X
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Zusammenfassung:Pregnant women (n = 859) in rural Cameroonian prenatal clinics were screened by two rapid human immunodeficiency virus (HIV) antibody tests (rapid tests [RT]) (Determine and Hema-Strip) using either whole blood or plasma. One additional RT (Capillus, HIV-CHEK, or Sero-Card) was used to resolve discordant results. RT results were compared with HIV-1 enzyme immunoassay (EIA) and Western blot (WB) results of matched dried blood spots (DBS) to assess the accuracy of HIV RTs. DBS EIA/WB identified 83 HIV antibody-reactive, 763 HIV antibody-nonreactive, and 13 indeterminate specimens. RT results were evaluated in serial (two consecutive tests) or parallel (two simultaneous tests) testing algorithms. A serial algorithm using Determine and Hema-Strip yielded sensitivity and specificity results of 97.6% and 99.7%, respectively, whereas a parallel RT algorithm using Determine plus a second RT produced a sensitivity and specificity of 100% and 99.7%, respectively. HIV RTs provide excellent alternatives for identifying HIV infection, and their field performance could be monitored using DBS testing strategies.
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ISSN:1071-412X
DOI:10.1128/CDLI.12.7.855-860.2005