Interactions of Human Dermal Dendritic Cells and Langerhans Cells Treated with Hyalomma Tick Saliva with Crimean-Congo Hemorrhagic Fever Virus

Crimean-Congo hemorrhagic fever virus is one the most important and wide spread tick-borne viruses. Very little is known about the transmission from the tick and the early aspects of pathogenesis. Here, we generate human cutaneous antigen presenting cells—dermal dendritic cells and Langerhans cells—...

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Veröffentlicht in:	Viruses Jg. 10; H. 7; S. 381
Hauptverfasser:	Rodriguez, Sergio E., McAuley, Alexander J., Gargili, Aysen, Bente, Dennis A.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Switzerland MDPI AG 20.07.2018 MDPI
Schlagworte:	Animals Antigen-presenting cells Antigens Arachnids Cell activation Cell adhesion & migration Cell Migration Assays Cell Movement Chemokines - immunology Crimean hemorrhagic fever Crimean-Congo hemorrhagic fever Crimean-Congo hemorrhagic fever virus Cytokines - immunology Dendritic cells Dendritic Cells - immunology Dendritic Cells - virology dermal dendritic cells Feeding Fever Gene Expression Profiling Hemorrhage Hemorrhagic Fever Virus, Crimean-Congo Hemorrhagic Fever, Crimean - immunology human cutaneous immune response Humans Hyalomma marginatum Immune response Immunologic Factors - immunology Immunology Infections Ixodidae - chemistry Ixodidae - virology Laboratory animals Langerhans cells Langerhans Cells - immunology Langerhans Cells - virology Leukocyte migration Pathogenesis Penicillin Progenitor cells Saliva Saliva - chemistry Salivary gland Salivary Glands - chemistry Skin Skin - cytology Skin - immunology Stem cells tick-borne virus tick-virus-host interface Tissue Extracts - chemistry Umbilical cord Viruses Canada United States > US Turkey Hyalomma marginatum human cutaneous immune response tick-borne virus tick-virus-host interface Langerhans cells dermal dendritic cells Crimean-Congo hemorrhagic fever Crimean-Congo hemorrhagic fever virus
ISSN:	1999-4915, 1999-4915
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Zusammenfassung:	Crimean-Congo hemorrhagic fever virus is one the most important and wide spread tick-borne viruses. Very little is known about the transmission from the tick and the early aspects of pathogenesis. Here, we generate human cutaneous antigen presenting cells—dermal dendritic cells and Langerhans cells—from umbilical cord progenitor cells. In order to mimic the environment created during tick feeding, tick salivary gland extract was generated from semi-engorged Hyalomma marginatum ticks. Our findings indicate that human dermal dendritic cells and Langerhans cells are susceptible and permissive to Crimean-Congo hemorrhagic fever virus infection, however, to different degrees. Infection leads to cell activation and cytokine/chemokine secretion, although these responses vary between the different cell types. Hyalomma marginatum salivary gland extract had minimal effect on cell responses, with some synergy with viral infection with respect to cytokine secretion. However, salivary gland extract appeared to inhibit antigen presenting cells (APCs) migration. Based on the findings here we hypothesize that human dermal dendritic cells and Langerhans cells serve as early target cells. Rather affecting Crimean-Congo hemorrhagic fever virus replication, tick saliva likely immunomodulates and inhibits migration of these APCs from the feeding site.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work. Current Address: CSIRO Australian Animal Health Laboratory, 5 Portarlington Road, East Geelong, VIC 3219, Australia.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v10070381