Identification and Characterization of a New Strain of Sunflower chlorotic mottle virus, a Potyvirus Infecting Asteraceae in Argentina

Sunflower (Helianthus annuus L.) plants with chlorotic ringspot symptoms were observed during the 2005-2006 crop season in the Southeast of the province of Buenos Aires, Argentina. Symptomatic plants were tested by biological, serological and molecular assays, and the virus isolated was identified a...

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Vydáno v:Journal of phytopathology Ročník 158; číslo 7-8; s. 536 - 541
Hlavní autoři: Giolitti, Fabian J, Bejerman, Nicolas E, deBreuil, Soledad, Lenardon, Sergio L
Médium: Journal Article
Jazyk:angličtina
Vydáno: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2010
Blackwell Publishing Ltd
Blackwell
Témata:
antiserum
Argentina
Asteraceae
Biological and medical sciences
Capsid protein
chlorotic ringspot (CRS)
coat proteins
CP gene
Crops
Enzymes
Fundamental and applied biological sciences. Psychology
genes
Helianthus
Helianthus annuus
Inclusion bodies
Nucleotides
Phytopathology. Animal pests. Plant and forest protection
Plant viruses
Plant viruses and viroids
Potyvirus
restriction endonucleases
sequence analysis
sunflower
Sunflower chlorotic mottle virus
viruses
Argentina
South America
America
Plant pathology
Argentina
Plant pathogen
Sunflower chlorotic mottle virus
Identification
capsid protein
Compositae
Protein
chlorotic ringspot (CRS)
sunflower
Strain
Virus
Plant
Potato virus Y
Dicotyledones
Angiospermae
Spermatophyta
Potyvirus
Capsid
Potyviridae
ISSN:0931-1785, 1439-0434
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Shrnutí:Sunflower (Helianthus annuus L.) plants with chlorotic ringspot symptoms were observed during the 2005-2006 crop season in the Southeast of the province of Buenos Aires, Argentina. Symptomatic plants were tested by biological, serological and molecular assays, and the virus isolated was identified as a potyvirus closely related to Sunflower chlorotic mottle virus, common isolate (SuCMoV-C), the most prevalent virus in sunflower crops in the country. Infected plants were serologically positive when probed with a SuCMoV-C antiserum. In the 3′-terminus region, 1304 nucleotides (nt) were sequenced, and it includes the C-terminal region of the nuclear inclusion b protein (NIb) gene (240 nt), the whole capsid protein (CP) gene (807 nt) and a 3′-non-coding region (3′-NCR) with 257 nt excluding the poly (A) tail. The CP of the Sunflower potyvirus causing chlorotic ringspot (CRS) shared 94.8% aa identity with SuCMoV-C and 89.2% with SuCMoV-Zi. The 3′-NCR shared 94.2% nt sequence identity with SuCMoV-C. A RT-PCR/RFLP assay with PvuII and EcoRV restriction enzymes successfully differentiated SuCMoV-C and the virus isolate causing CRS symptoms. This potyvirus was identified as a new SuCMoV strain, provisionally designated SuCMoV-CRS.
Bibliografie:http://dx.doi.org/10.1111/j.1439-0434.2009.01656.x
ArticleID:JPH1656
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ObjectType-Article-1
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ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2009.01656.x