Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates

We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher b...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Vol. 27; no. 6; pp. 1255 - 1264
Main Authors: Sparman, Michelle, Dighe, Vikas, Sritanaudomchai, Hathaitip, Ma, Hong, Ramsey, Cathy, Pedersen, Darlene, Clepper, Lisa, Nighot, Prashant, Wolf, Don, Hennebold, Jon, Mitalipov, Shoukhrat
Format: Journal Article
Language:English
Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2009
Subjects:
Animals
Cell Differentiation
Cellular Reprogramming - genetics
Embryonic stem cells
Embryonic Stem Cells - cytology
Epigenesis, Genetic
Female
Fibroblasts - cytology
Gene Expression
Macaca mulatta
Nuclear transfer
Nuclear Transfer Techniques
Oocytes - cytology
Pluripotent Stem Cells - cytology
Primates
Reprogramming
Reverse Transcriptase Polymerase Chain Reaction
ISSN:1066-5099, 1549-4918, 1549-4918
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Summary:We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor cells. Two ES cell lines were isolated using adult female rhesus macaque skin fibroblasts as nuclear donors and oocytes retrieved from one female, following a single controlled ovarian stimulation. In addition to routine pluripotency tests involving in vitro and in vivo differentiation into various somatic cell types, primate ES cells derived from reprogrammed somatic cells were also capable of contributing to cells expressing markers of germ cells. Moreover, imprinted gene expression, methylation, telomere length, and X‐inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte‐specific factors. STEM CELLS 2009;27:1255–1264
Bibliography:First published online in S
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March 12, 2009
Author contributions: M.S. and V.D.: conception and design, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing; H.S., H.M., C.R., D.P., L.C.: provision of study material, collection and/or assembly of data, manuscript writing; P.N.: collection and/or assembly of data; D.W.: data analysis and interpretation, manuscript writing; J.H.: collection and/or assembly of data, manuscript writing; S.M.: conception and design, financial support, administrative support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript.
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ISSN:1066-5099
1549-4918
1549-4918
DOI:10.1002/stem.60