Custom human endogenous retroviruses dedicated microarray identifies self-induced HERV-W family elements reactivated in testicular cancer upon methylation control

Endogenous retroviruses (ERVs) are an inherited part of the eukaryotic genomes, and represent ~400 000 loci in the human genome. Human endogenous retroviruses (HERVs) can be divided into distinct families, composed of phylogenetically related but structurally heterogeneous elements. The majority of...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids research Jg. 38; H. 7; S. 2229 - 2246
Hauptverfasser: Gimenez, Juliette, Montgiraud, Cécile, Pichon, Jean-Philippe, Bonnaud, Bertrand, Arsac, Maud, Ruel, Karine, Bouton, Olivier, Mallet, François
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Oxford University Press 01.04.2010
Schlagworte:
DNA
DNA Methylation
Endogenous Retroviruses - genetics
Endogenous Retroviruses - metabolism
Epigenesis, Genetic
Gene Expression Profiling - methods
gene overexpression
Gene Regulation, Chromatin and Epigenetics
Genetic Loci
genome
Humans
inheritance (genetics)
loci
Male
methylation
microarray technology
neoplasms
Oligonucleotide Array Sequence Analysis - methods
pathophysiology
phylogeny
placenta
Retroviridae
Retrovirus
Reverse Transcriptase Polymerase Chain Reaction
RNA Splicing
Terminal Repeat Sequences
testes
Testicular Neoplasms - virology
transcription (genetics)
Transcription, Genetic
transcriptome
ISSN:0305-1048, 1362-4962, 1362-4962
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Endogenous retroviruses (ERVs) are an inherited part of the eukaryotic genomes, and represent ~400 000 loci in the human genome. Human endogenous retroviruses (HERVs) can be divided into distinct families, composed of phylogenetically related but structurally heterogeneous elements. The majority of HERVs are silent in most physiological contexts, whereas a significant expression is observed in pathological contexts, such as cancers. Owing to their repetitive nature, few of the active HERV elements have been accurately identified. In addition, there are no criteria defining the active promoters among HERV long-terminal repeats (LTRs). Hence, it is difficult to understand the HERV (de)regulation mechanisms and their implication on the physiopathology of the host. We developed a microarray to specifically detect the LTR-containing transcripts from the HERV-H, HERV-E, HERV-W and HERV-K(HML-2) families. HERV transcriptome was analyzed in the placenta and seven normal/tumoral match-pair samples. We identified six HERV-W loci overexpressed in testicular cancer, including a usually placenta-restricted transcript of ERVWE1. For each locus, specific overexpression was confirmed by quantitative RT-PCR, and comparison of the activity of U3 versus U5 regions suggested a U3-promoted transcription coupled with 5'R initiation. The analysis of DNA from tumoral versus normal tissue revealed that hypomethylation of U3 promoters in tumors is a prerequisite for their activation.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
The authors wish it to be known that, in their opinion, the first four authors should be regarded as joint First Authors.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkp1214