Protective immunity induced by oral immunization with a rotavirus DNA vaccine encapsulated in microparticles

DNA vaccines are usually given by intramuscular injection or by gene gun delivery of DNA-coated particles into the epidermis. Induction of mucosal immunity by targeting DNA vaccines to mucosal surfaces may offer advantages, and an oral vaccine could be effective for controlling infections of the gut...

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Bibliographic Details
Published in:Journal of virology Vol. 72; no. 7; p. 5757
Main Authors: Chen, S C, Jones, D H, Fynan, E F, Farrar, G H, Clegg, J C, Greenberg, H B, Herrmann, J E
Format: Journal Article
Language:English
Published: United States 01.07.1998
Subjects:
Administration, Oral
Animals
Antibodies, Viral - blood
Antigens, Viral
Capsid - genetics
Capsid - immunology
Capsid Proteins
Immunization
Immunoglobulin A - analysis
Mice
Mice, Inbred BALB C
Rotavirus - immunology
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
ISSN:0022-538X
Online Access:Get more information
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Summary:DNA vaccines are usually given by intramuscular injection or by gene gun delivery of DNA-coated particles into the epidermis. Induction of mucosal immunity by targeting DNA vaccines to mucosal surfaces may offer advantages, and an oral vaccine could be effective for controlling infections of the gut mucosa. In a murine model, we obtained protective immune responses after oral immunization with a rotavirus VP6 DNA vaccine encapsulated in poly(lactide-coglycolide) (PLG) microparticles. One dose of vaccine given to BALB/c mice elicited both rotavirus-specific serum antibodies and intestinal immunoglobulin A (IgA). After challenge at 12 weeks postimmunization with homologous rotavirus, fecal rotavirus antigen was significantly reduced compared with controls. Earlier and higher fecal rotavirus-specific IgA responses were noted during the peak period of viral shedding, suggesting that protection was due to specific mucosal immune responses. The results that we obtained with PLG-encapsulated rotavirus VP6 DNA are the first to demonstrate protection against an infectious agent elicited after oral administration of a DNA vaccine.
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ISSN:0022-538X
DOI:10.1128/jvi.72.7.5757-5761.1998