Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization

Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of so...

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Vydáno v:Frontiers in plant science Ročník 13; s. 920522
Hlavní autoři: Jia, Hongchang, Liang, Xin, Zhang, Lixin, Zhang, Jinmei, Sapey, Enoch, Liu, Xianyuan, Sun, Yanhui, Sun, Shi, Yan, Hongrui, Lu, Wencheng, Han, Tianfu
Médium: Journal Article
Jazyk:angličtina
Vydáno: Lausanne Frontiers Media SA 30.06.2022
Frontiers Media S.A
Témata:
Agronomy
Dehydration
Flowers & plants
germination rate
Germplasm
Humidity
Hybridization
Inbreeding
Lamps
Low temperature
off-site hybridization
Plant breeding
Plant Science
Pollen
Preservation
soybean
Soybeans
ultra-low temperature preservation
Beijing China
China
ISSN:1664-462X, 1664-462X
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Abstract Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6–10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at −196 or −80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
AbstractList Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6-10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at -196 or -80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6-10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at -196 or -80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6–10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at −196 or −80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6–10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at −196 or −80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
Author Liang, Xin
Lu, Wencheng
Jia, Hongchang
Zhang, Jinmei
Sun, Yanhui
Yan, Hongrui
Liu, Xianyuan
Sapey, Enoch
Zhang, Lixin
Sun, Shi
Han, Tianfu
AuthorAffiliation 2 Heihe Branch, Heilongjiang Academy of Agricultural Sciences , Heihe , China
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing , China
3 Council for Scientific and Industrial Research (CSIR)-Oil Palm Research Institute , Kade , Ghana
AuthorAffiliation_xml – name: 2 Heihe Branch, Heilongjiang Academy of Agricultural Sciences , Heihe , China
– name: 3 Council for Scientific and Industrial Research (CSIR)-Oil Palm Research Institute , Kade , Ghana
– name: 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences , Beijing , China
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Reviewed by: Zhihui Shan, Oil Crops Research Institute (CAAS), China; Shouping Yang, Nanjing Agricultural University, China
These authors have contributed equally to this work
Edited by: Deyue Yu, Nanjing Agricultural University, China
This article was submitted to Technical Advances in Plant Science, a section of the journal Frontiers in Plant Science
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Snippet Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen...
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StartPage 920522
SubjectTerms Agronomy
Dehydration
Flowers & plants
germination rate
Germplasm
Humidity
Hybridization
Inbreeding
Lamps
Low temperature
off-site hybridization
Plant breeding
Plant Science
Pollen
Preservation
soybean
Soybeans
ultra-low temperature preservation
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Title Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization
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