Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization

Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of so...

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Published in:Frontiers in plant science Vol. 13; p. 920522
Main Authors: Jia, Hongchang, Liang, Xin, Zhang, Lixin, Zhang, Jinmei, Sapey, Enoch, Liu, Xianyuan, Sun, Yanhui, Sun, Shi, Yan, Hongrui, Lu, Wencheng, Han, Tianfu
Format: Journal Article
Language:English
Published: Lausanne Frontiers Media SA 30.06.2022
Frontiers Media S.A
Subjects:
Agronomy
Dehydration
Flowers & plants
germination rate
Germplasm
Humidity
Hybridization
Inbreeding
Lamps
Low temperature
off-site hybridization
Plant breeding
Plant Science
Pollen
Preservation
soybean
Soybeans
ultra-low temperature preservation
Beijing China
China
ISSN:1664-462X, 1664-462X
Online Access:Get full text
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Summary:Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6–10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at −196 or −80°C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
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Reviewed by: Zhihui Shan, Oil Crops Research Institute (CAAS), China; Shouping Yang, Nanjing Agricultural University, China
These authors have contributed equally to this work
Edited by: Deyue Yu, Nanjing Agricultural University, China
This article was submitted to Technical Advances in Plant Science, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.920522