Escherichia ruysiae May Serve as a Reservoir of Antibiotic Resistance Genes across Multiple Settings and Regions
Escherichia ruysiae is a recently described species of the cryptic clades III and IV of the genus Escherichia and is commonly found in animals and the environment. This work highlights the zoonotic potential of E. ruysiae , as it has been shown to colonize the human intestinal tract. Gut colonizatio...
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| Veröffentlicht in: | Microbiology spectrum Jg. 11; H. 4; S. e0175323 |
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| Hauptverfasser: | , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
United States
American Society for Microbiology
17.08.2023
|
| Schlagworte: | |
| ISSN: | 2165-0497, 2165-0497 |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | Escherichia ruysiae
is a recently described species of the cryptic clades III and IV of the genus
Escherichia
and is commonly found in animals and the environment. This work highlights the zoonotic potential of
E. ruysiae
, as it has been shown to colonize the human intestinal tract.
Gut colonization with multidrug-resistant
Enterobacterale
s (MDR-
Ent
) has reached worrisome levels worldwide. In this context,
Escherichia ruysiae
is a recently described species mostly found in animals. However, its spread and impact on humans is poorly understood. A stool sample from a healthy individual living in India was screened for the presence of MDR-
Ent
using culture-based methods. Colonies were routinely identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and phenotypically characterized by broth microdilution. Illumina and Nanopore whole-genome sequencing (WGS) platforms were implemented to generate a complete assembly.
E. ruysiae
genomes deposited in international databases were used for a core genome phylogenetic analysis. An extended-spectrum β-lactamase (ESBL)-producing
E. coli
strain (S1-IND-07-A) was isolated from the stool. WGS confirmed that S1-IND-07-A was indeed
E. ruysiae
, belonged to sequence type 5792 (ST5792), core genome (cg) ST89059, serotype O13/O129−:H56-like, clade IV phylogroup, and possessed five virulence factors. A copy of
bla
CTX-M-15
and five other antimicrobial resistance genes (ARGs) were detected in a conjugative IncB/O/K/Z plasmid. A database search identified 70 further
E. ruysiae
strains from 16 countries (44, 15, and 11 strains isolated from animals, the environment, and humans, respectively). The core genome phylogeny revealed five major STs: ST6467, ST8084, ST2371, ST9287, and ST5792. Three out of the seventy strains possessed important ARGs: OTP1704 (
bla
CTX-M-14
; ST6467), SN1013-18 (
bla
CTX-M-15
; ST5792), and CE1758 (
bla
CMY-2
; ST7531). These strains were of human, environmental, and wild animal origin, respectively.
E. ruysiae
may acquire clinically important ARGs and transmit them to other species. Due to its zoonotic potential, further efforts are needed to improve routine detection and surveillance across One Health settings.
IMPORTANCE
Escherichia ruysiae
is a recently described species of the cryptic clades III and IV of the genus
Escherichia
and is commonly found in animals and the environment. This work highlights the zoonotic potential of
E. ruysiae
, as it has been shown to colonize the human intestinal tract. Importantly,
E. ruysiae
may be associated with conjugative plasmids carrying clinically relevant antibiotic resistance genes. Therefore, it is important to closely monitor this species. Overall, this study highlights the need for improved identification of
Escherichia
species and continued surveillance of zoonotic pathogens in One Health settings. |
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| Bibliographie: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 The authors declare no conflict of interest. |
| ISSN: | 2165-0497 2165-0497 |
| DOI: | 10.1128/spectrum.01753-23 |