The influence of sweet pepper pectin structural characteristics on cytokine secretion by THP-1 macrophages

Pectins can modulate the biological responses interacting directly with immune cells. The observed responses can strongly be affected by polysaccharide structural features. We analyzed the intrinsic activation capacity of native and modified sweet pepper pectin on cytokine secretion by THP-1 macroph...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Food research international Ročník 102; s. 588 - 594
Hlavní autori: do Nascimento, Georgia Erdmann, Winnischofer, Sheila Maria Brochado, Ramirez, Marcel Ivan, Iacomini, Marcello, Cordeiro, Lucimara Mach Côrtes
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Canada Elsevier Ltd 01.12.2017
Predmet:
acid hydrolysis
Cytokine secretion
esterification
Immunomodulatory activity
inflammation
interleukin-10
interleukin-1beta
lipopolysaccharides
macrophages
molecular weight
pectins
secretion
Structure-function relationship
Sweet pepper pectin
sweet peppers
THP-1 macrophages
tumor necrosis factor-alpha
viability
Cytokine secretion
Immunomodulatory activity
THP-1 macrophages
Structure-function relationship
Sweet pepper pectin
ISSN:0963-9969, 1873-7145, 1873-7145
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Pectins can modulate the biological responses interacting directly with immune cells. The observed responses can strongly be affected by polysaccharide structural features. We analyzed the intrinsic activation capacity of native and modified sweet pepper pectin on cytokine secretion by THP-1 macrophages as well as compare their effects in the presence of lipopolysaccharide. Modified pectin was obtained by partial acid hydrolysis which promoted the removal of side chains as well as the reduction of molecular weight and the degree of methyl esterification of native pectin. The results showed that both fractions had no effect on THP-1 viability. Native pectin at 300μg/mL increased TNF-α, IL-1β and IL-10 cytokine secretion by THP-1 macrophages. However, in the presence of lipopolysaccharide, it can attenuate the inflammatory response by reducing the production of the pro-inflammatory cytokines TNF-α and IL-1β and increasing the anti-inflammatory cytokine IL-10, as well as decreasing the TNF-α/IL-10 and IL-1β/IL-10 ratios. The structural modifications caused by acid hydrolysis affected the intrinsic activation capacity of native pectin to modulate the cytokines secretion. These results indicate that degree of methyl esterification, molecular weight and presence of side chains are important structural features of pectins involved in the modulation of cytokine secretion by THP-1 macrophages. [Display omitted] •The impact of structural differences of pectin on cytokine secretion was evaluated.•Removal of sweet pepper pectin side chains by partial acid hydrolysis was performed.•Sweet pepper pectins stimulated TNF-α, IL-1β and IL-10 secretion by THP-1 macrophage.•The removal of pectin side chains altered the cytokine secretion profile.•Both native and modified pectins attenuated the inflammatory response triggered by LPS stimulation.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0963-9969
1873-7145
1873-7145
DOI:10.1016/j.foodres.2017.09.037