Mesenchymal stem cells from iPS cells facilitate periodontal regeneration

Mesenchymal stem cells (MSC) have been considered as a potential therapy for the treatment of periodontal defects arising from periodontitis. However, issues surrounding their accessibility and proliferation in culture significantly limit their ability to be used as a mainstream treatment approach....

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Bibliographic Details
Published in:Journal of dental research Vol. 92; no. 9; p. 833
Main Authors: Hynes, K, Menicanin, D, Han, J, Marino, V, Mrozik, K, Gronthos, S, Bartold, P M
Format: Journal Article
Language:English
Published: United States 01.09.2013
Subjects:
Alveolar Bone Loss - surgery
Animals
Antimetabolites
Bone Regeneration - physiology
Bromodeoxyuridine
Calcification, Physiologic - physiology
Cell Culture Techniques
Cell Differentiation - physiology
Cell Lineage - physiology
Disease Models, Animal
Flow Cytometry
Humans
Image Processing, Computer-Assisted - methods
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stromal Cells - physiology
Osteoblasts - pathology
Osteocytes - pathology
Osteogenesis - physiology
Periodontal Diseases - therapy
Periodontium - pathology
Periodontium - physiopathology
Pluripotent Stem Cells - physiology
Rats
Rats, Nude
Regeneration - physiology
MSC derivation
tissue regeneration
cell differentiation
induced pluripotent stem cells
periodontal fenestration defect
periodontal disease
ISSN:1544-0591, 1544-0591
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Summary:Mesenchymal stem cells (MSC) have been considered as a potential therapy for the treatment of periodontal defects arising from periodontitis. However, issues surrounding their accessibility and proliferation in culture significantly limit their ability to be used as a mainstream treatment approach. It is therefore important that alternative, easily accessible, and safe populations of stem cells be identified. Controlled induction of induced pluripotent stem cells (iPSC) into MSC-like cells is emerging as an attractive source for obtaining large populations of stem cells for regenerative medicine. We have successfully induced iPSC to differentiate into MSC-like cells. The MSC-like cells generated satisfied the International Society of Cellular Therapy's minimal criteria for defining multipotent MSC, since they had plastic adherent properties, expressed key MSC-associated markers, and had the capacity to undergo tri-lineage differentiation. Importantly, the resulting iPSC-MSC-like cells also had the capacity, when implanted into periodontal defects, to significantly increase the amount of regeneration and newly formed mineralized tissue present. Our results demonstrate, for the first time, that MSC derived from iPSC have the capacity to aid periodontal regeneration and are a promising source of readily accessible stem cells for use in the clinical treatment of periodontitis.
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ISSN:1544-0591
1544-0591
DOI:10.1177/0022034513498258