Developing human pluripotent stem cell-based cerebral organoids with a controllable microglia ratio for modeling brain development and pathology

Microglia play critical roles in brain development, homeostasis, and disease. Microglia in animal models cannot accurately model human microglia due to notable transcriptomic and functional differences between human and other animal microglia. Incorporating human pluripotent stem cell (hPSC)-derived...

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Veröffentlicht in:Stem cell reports Jg. 16; H. 8; S. 1923 - 1937
Hauptverfasser: Xu, Ranjie, Boreland, Andrew J., Li, Xiaoxi, Erickson, Caroline, Jin, Mengmeng, Atkins, Colm, Pang, Zhiping P., Daniels, Brian P., Jiang, Peng
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 10.08.2021
Elsevier
Schlagworte:
Brain - cytology
Brain - metabolism
Cell Differentiation - genetics
Cell Line
Cells, Cultured
cerebral organoid
Coculture Techniques
Gene Expression Profiling - methods
human iPSC
human pluripotent stem cell
Humans
Induced Pluripotent Stem Cells - metabolism
microglia
Microglia - cytology
Microglia - metabolism
Microglia - virology
Models, Neurological
Neural Stem Cells - metabolism
Organoids - cytology
Organoids - metabolism
Organoids - virology
Pluripotent Stem Cells - metabolism
Synapses - genetics
synaptic pruning
Zika virus
Zika Virus - physiology
Zika Virus Infection - metabolism
Zika Virus Infection - virology
human iPSC
Zika virus
microglia
human pluripotent stem cell
cerebral organoid
synaptic pruning
ISSN:2213-6711, 2213-6711
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Zusammenfassung:Microglia play critical roles in brain development, homeostasis, and disease. Microglia in animal models cannot accurately model human microglia due to notable transcriptomic and functional differences between human and other animal microglia. Incorporating human pluripotent stem cell (hPSC)-derived microglia into brain organoids provides unprecedented opportunities to study human microglia. However, an optimized method that integrates appropriate amounts of microglia into brain organoids at a proper time point, resembling in vivo brain development, is still lacking. Here, we report a new brain region-specific, microglia-containing organoid model by co-culturing hPSC-derived primitive neural progenitor cells and primitive macrophage progenitors. In the organoids, the number of human microglia can be controlled, and microglia exhibit phagocytic activity and synaptic pruning function. Furthermore, human microglia respond to Zika virus infection of the organoids. Our findings establish a new microglia-containing brain organoid model that will serve to study human microglial function in a variety of neurological disorders. •Co-culturing hPSC-derived PMPs and pNPCs generates microglia-containing organoids•The ratio of human microglia in the organoids can be controlled•Human microglia engulf NPCs, apoptotic cells, and synapses in the organoids•ZIKV causes excessive synaptic pruning by human microglia in the organoids In this article, Jiang and colleagues developed a new brain region-specific, microglia-containing organoid model by co-culturing hPSC-derived primitive neural progenitor cells and primitive macrophage progenitors. In these organoids, appropriate amounts of human microglia integrate into brain organoids at a proper time point, resembling in vivo brain development. Human microglia exhibit phagocytic and synaptic pruning functions and respond to ZIKV infection.
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ISSN:2213-6711
2213-6711
DOI:10.1016/j.stemcr.2021.06.011