The expression of prostate stem cell antigen in human clear cell renal cell carcinoma: a quantitative reverse transcriptase‐polymerase chain reaction analysis

OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA...

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Hauptverfasser: ELSAMMAN, ESSAM M., FUKUMORI, TOMOHARU, TANIMOTO, SHUJI, NAKANISHI, RYOICHI, TAKAHASHI, MASAYUKI, TOIDA, KAZUNORI, KANAYAMA, HIRO‐OMI
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Veröffentlicht: Oxford, UK Blackwell Publishing Ltd 01.09.2006
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Abstract OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC‐RCC. PATIENTS AND METHODS We quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK‐1, Caki‐1, and Caki‐2) and in 154 surgical tissue samples (81 from CC‐RCC, 73 from normal kidney) using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light‐microscopy. RESULTS PSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC‐RCC than in normal kidney tissue samples (P < 0.001), in G2‐G3 than in G1 tumours (P = 0.028), and in advanced disease (T3‐T4) than in organ‐confined (T1‐T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC‐RCC than in normal kidney, and in advanced‐disease high‐grade tumours than in organ‐confined low‐grade tumours. CONCLUSIONS A significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC‐RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC‐RCC.
AbstractList To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC-RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC-RCC.OBJECTIVESTo analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC-RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC-RCC.We quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK-1, Caki-1, and Caki-2) and in 154 surgical tissue samples (81 from CC-RCC, 73 from normal kidney) using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light-microscopy.PATIENTS AND METHODSWe quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK-1, Caki-1, and Caki-2) and in 154 surgical tissue samples (81 from CC-RCC, 73 from normal kidney) using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light-microscopy.PSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC-RCC than in normal kidney tissue samples (P < 0.001), in G2-G3 than in G1 tumours (P = 0.028), and in advanced disease (T3-T4) than in organ-confined (T1-T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC-RCC than in normal kidney, and in advanced-disease high-grade tumours than in organ-confined low-grade tumours.RESULTSPSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC-RCC than in normal kidney tissue samples (P < 0.001), in G2-G3 than in G1 tumours (P = 0.028), and in advanced disease (T3-T4) than in organ-confined (T1-T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC-RCC than in normal kidney, and in advanced-disease high-grade tumours than in organ-confined low-grade tumours.A significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC-RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC-RCC.CONCLUSIONSA significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC-RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC-RCC.
To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC-RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC-RCC. We quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK-1, Caki-1, and Caki-2) and in 154 surgical tissue samples (81 from CC-RCC, 73 from normal kidney) using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light-microscopy. PSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC-RCC than in normal kidney tissue samples (P < 0.001), in G2-G3 than in G1 tumours (P = 0.028), and in advanced disease (T3-T4) than in organ-confined (T1-T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC-RCC than in normal kidney, and in advanced-disease high-grade tumours than in organ-confined low-grade tumours. A significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC-RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC-RCC.
OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC‐RCC. PATIENTS AND METHODS We quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK‐1, Caki‐1, and Caki‐2) and in 154 surgical tissue samples (81 from CC‐RCC, 73 from normal kidney) using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light‐microscopy. RESULTS PSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC‐RCC than in normal kidney tissue samples (P < 0.001), in G2‐G3 than in G1 tumours (P = 0.028), and in advanced disease (T3‐T4) than in organ‐confined (T1‐T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC‐RCC than in normal kidney, and in advanced‐disease high‐grade tumours than in organ‐confined low‐grade tumours. CONCLUSIONS A significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC‐RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC‐RCC.
Author ELSAMMAN, ESSAM M.
FUKUMORI, TOMOHARU
TAKAHASHI, MASAYUKI
TOIDA, KAZUNORI
NAKANISHI, RYOICHI
KANAYAMA, HIRO‐OMI
TANIMOTO, SHUJI
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ISSN 1464-4096
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Issue 3
Keywords Histological grading
Nephrology
Prognosis
RNA-directed DNA polymerase
PSCA
Tumoral marker
real-time RT-PCR
Carcinogenesis
Urology
kidney
Cancerology
Histopathology
Prostate stem cell antigen
Grawitz tumor
Reverse transcription polymerase chain reaction
Quantitative analysis
Kidney disease
Human
Urinary system disease
Enzyme
Transferases
Malignant tumor
Real time
Anatomic pathology
Nucleotidyltransferases
Clear cell carcinoma
Kidney cancer
Molecular biology
Language English
License CC BY 4.0
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Snippet OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship...
To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC-RCC) and its relationship with...
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SubjectTerms Antigens, Neoplasm
Biological and medical sciences
Carcinoma, Renal Cell - metabolism
Cell Line, Tumor
clear cell carcinoma
Female
Gene Expression
GPI-Linked Proteins
Humans
kidney
Kidney Neoplasms - metabolism
Kidneys
Male
Medical sciences
Membrane Glycoproteins - metabolism
Middle Aged
Neoplasm Proteins - metabolism
Nephrology. Urinary tract diseases
Prognosis
PSCA
real‐time RT‐PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
Tumors of the urinary system
Title The expression of prostate stem cell antigen in human clear cell renal cell carcinoma: a quantitative reverse transcriptase‐polymerase chain reaction analysis
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1464-410X.2006.06350.x
https://www.ncbi.nlm.nih.gov/pubmed/16925770
https://www.proquest.com/docview/68778630
Volume 98
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