The expression of prostate stem cell antigen in human clear cell renal cell carcinoma: a quantitative reverse transcriptase‐polymerase chain reaction analysis

OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA...

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Vydané v:BJU international Ročník 98; číslo 3; s. 668 - 673
Hlavní autori: ELSAMMAN, ESSAM M., FUKUMORI, TOMOHARU, TANIMOTO, SHUJI, NAKANISHI, RYOICHI, TAKAHASHI, MASAYUKI, TOIDA, KAZUNORI, KANAYAMA, HIRO‐OMI
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Oxford, UK Blackwell Publishing Ltd 01.09.2006
Blackwell
Predmet:
Antigens, Neoplasm
Biological and medical sciences
Carcinoma, Renal Cell - metabolism
Cell Line, Tumor
clear cell carcinoma
Female
Gene Expression
GPI-Linked Proteins
Humans
kidney
Kidney Neoplasms - metabolism
Kidneys
Male
Medical sciences
Membrane Glycoproteins - metabolism
Middle Aged
Neoplasm Proteins - metabolism
Nephrology. Urinary tract diseases
Prognosis
PSCA
real‐time RT‐PCR
Reverse Transcriptase Polymerase Chain Reaction - methods
Tumors of the urinary system
Histological grading
Nephrology
Prognosis
RNA-directed DNA polymerase
PSCA
Tumoral marker
real-time RT-PCR
Carcinogenesis
Urology
kidney
Cancerology
Histopathology
Prostate stem cell antigen
Grawitz tumor
Reverse transcription polymerase chain reaction
Quantitative analysis
Kidney disease
Human
Urinary system disease
Enzyme
Transferases
Malignant tumor
Real time
Anatomic pathology
Nucleotidyltransferases
Clear cell carcinoma
Kidney cancer
Molecular biology
ISSN:1464-4096, 1464-410X
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Shrnutí:OBJECTIVES To analyse the gene expression level of prostate stem cell antigen (PSCA) in human clear cell renal cell carcinoma (CC‐RCC) and its relationship with conventional clinicopathological manifestations, to evaluate its prognostic value for patient outcome, and to determine the effect of PSCA on the progression of CC‐RCC. PATIENTS AND METHODS We quantified PSCA mRNA level in human RCC cell lines (ACHN, A704, KPK‐1, Caki‐1, and Caki‐2) and in 154 surgical tissue samples (81 from CC‐RCC, 73 from normal kidney) using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). The findings were analysed in relation to clinicopathological factors. Immunohistochemical expression was examined using confocal laser scanning light‐microscopy. RESULTS PSCA was overexpressed in all RCC cell lines. PSCA mRNA levels were significantly higher in CC‐RCC than in normal kidney tissue samples (P < 0.001), in G2‐G3 than in G1 tumours (P = 0.028), and in advanced disease (T3‐T4) than in organ‐confined (T1‐T2) tumours (P = 0.016). There was significantly higher PSCA mRNA expression in patients with M1 than in those with M0 disease (P = 0.029). Patients in whom the lesions had high PSCA expression levels had a significantly worse prognosis than those with low PSCA expression levels (P = 0.044). Using immunohistochemical analysis there was markedly greater PSCA expression in CC‐RCC than in normal kidney, and in advanced‐disease high‐grade tumours than in organ‐confined low‐grade tumours. CONCLUSIONS A significant correlation was detected in the gene expression level of PSCA with histological grade, clinicopathological stage and prognosis in CC‐RCC. Our data indicate that PSCA is associated with carcinogenesis and progression of CC‐RCC.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1464-4096
1464-410X
DOI:10.1111/j.1464-410X.2006.06350.x