Phylogeography, Salinity Adaptations and Metabolic Potential of the Candidate Division KB1 Bacteria Based on a Partial Single Cell Genome

Deep-sea hypersaline anoxic basins and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that have been consistently observed in hypersaline microbi...

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Bibliographic Details
Published in:Frontiers in microbiology Vol. 7; p. 1266
Main Authors: Nigro, Lisa M., Hyde, Andrew S., MacGregor, Barbara J., Teske, Andreas
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 22.08.2016
Subjects:
Candidate Division KB1
Genome
hypersaline
Microbiology
Orca Basin
Phylogeography
genome
Candidate Division KB1
Orca Basin
hypersaline
phylogeography
ISSN:1664-302X, 1664-302X
Online Access:Get full text
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Summary:Deep-sea hypersaline anoxic basins and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that have been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis - previously developed based on (14)C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source.
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This article was submitted to Extreme Microbiology, a section of the journal Frontiers in Microbiology
Reviewed by: Ronald Oremland, United States Geological Survey, USA; Dimitry Y. Sorokin, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Russia
Edited by: Brian D. Lanoil, University of Alberta, Canada
Present address: Lisa M. Nigro, Center for Environmental Diagnostics and Bioremediation, Hal Marcus College of Science and Engineering, University of West Florida, Pensacola, FL, USA
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2016.01266