Preparation of ultrathin grafts for Descemet-stripping endothelial keratoplasty with a single microkeratome pass

We present a technique to achieve ultrathin Descemet-stripping automated endothelial keratoplasty (DSAEK). Using a simple method of controlling artificial anterior chamber pressure and drying the corneal surface, it was possible to thin the donor cornea at a rate of 11 μm a minute. When the donor co...

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Vydané v:Journal of cataract and refractive surgery Ročník 43; číslo 1; s. 12
Hlavní autori: Romano, Vito, Steger, Bernhard, Myneni, Jayavani, Batterbury, Mark, Willoughby, Colin E, Kaye, Stephen B
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.01.2017
Predmet:
Aged
Cell Count
Corneal Diseases - surgery
Descemet Stripping Endothelial Keratoplasty - methods
Endothelium, Corneal - diagnostic imaging
Endothelium, Corneal - transplantation
Humans
Middle Aged
Tissue and Organ Harvesting - methods
Tissue Donors
Tomography, Optical Coherence
ISSN:1873-4502, 1873-4502
On-line prístup:Zistit podrobnosti o prístupe
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Shrnutí:We present a technique to achieve ultrathin Descemet-stripping automated endothelial keratoplasty (DSAEK). Using a simple method of controlling artificial anterior chamber pressure and drying the corneal surface, it was possible to thin the donor cornea at a rate of 11 μm a minute. When the donor cornea was between 500 μm and 510 μm, a single pass was made using a 350 μm microkeratome head followed by a peripheral dissection. The resulting mean graft thickness was 83.2 μm ± 14.9 (SD) (range 50 to 98 μm) with a mean peripheral graft edge thickness of 106.8 ± 10.9 μm (range 90 to 120 μm). There were no surgical complications, and all grafts remained attached. This is a reliable method for preparing ultrathin donor corneal lenticules for DSAEK in the operating room or eye bank without using multiple microkeratome heads or risking double passes.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-4502
1873-4502
DOI:10.1016/j.jcrs.2016.12.009