OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice

Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 OsWRKY genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a sig...

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Vydáno v:Frontiers in plant science Ročník 12; s. 643011
Hlavní autoři: Li, Yanyun, Liao, Shuting, Mei, Pengying, Pan, Yueyun, Zhang, Yu, Zheng, Xiangzi, Xie, Yakun, Miao, Ying
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media S.A 22.03.2021
Témata:
biotic stress
flag leaf
OsWRKY93
Plant Science
rice
senescence
senescence
OsWRKY93
flag leaf
rice
biotic stress
ISSN:1664-462X, 1664-462X
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Shrnutí:Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 OsWRKY genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a significant altered expression level in the flag leaves during aging by using the reverse transcript quantitative PCR (RT-qPCR). Among them, the OsWRKY2 , OsWRKY14 , OsWRKY26 , OsWRKY69 , and OsWRKY93 members exhibited short-term alteration in transcriptional levels in response to Magnaporthe grisea infection. The CRISPR/Cas9-edited mutants of five genes were developed and confirmed, and a significant sensitivity to M. oryzae infection was observed in CRISPR OsWRKY93-edited lines; on the other hand, a significant resistance to M. oryzae infection was shown in the enhanced expression OsWRKY93 plants compared to mock plants; however, enhanced expression of other four genes have no significant affection. Interestingly, ROS accumulation was also increased in OsWRKY93 enhanced plants after flg22 treatment, compared with the controls, suggesting that OsWRKY93 is involved in PAMP-triggered immune response in rice. It indicated that OsWRKY93 was involved in both flag leaf senescence and in response to fungi attack.
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content type line 23
This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science
Edited by: Nam-Chon Paek, Seoul National University, South Korea
Reviewed by: Guodong Ren, Fudan University, China; Qian Qian, Chinese Academy of Agricultural Sciences, China
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2021.643011