Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen

Colony formation is the gold standard assay for determining reproductive cell death after radiation treatment, since effects on proliferation often do not reflect survival. We have developed a high-throughput radiosensitivity screening method based on clonogenicity and screened a siRNA library again...

Full description

Saved in:

Bibliographic Details
Published in:	Oncotarget Vol. 6; no. 8; p. 5978
Main Authors:	Tiwana, Gaganpreet S, Prevo, Remko, Buffa, Francesca M, Yu, Sheng, Ebner, Daniel V, Howarth, Alison, Folkes, Lisa K, Budwal, Balam, Chu, Kwun-Ye, Durrant, Lisa, Muschel, Ruth J, McKenna, W Gillies, Higgins, Geoff S
Format:	Journal Article
Language:	English
Published:	United States 20.03.2015
Subjects:	Cell Line, Tumor Colony-Forming Units Assay DNA Damage HCT116 Cells HeLa Cells High-Throughput Screening Assays Humans Neoplasms - metabolism Neoplasms - radiotherapy Pyrithiamine - pharmacology Radiation Tolerance Radiation-Sensitizing Agents - pharmacology Thiamin Pyrophosphokinase - metabolism Thiamine - metabolism Transfection thiamine tumor radiosensitivity TPK1 high-throughput screening
ISSN:	1949-2553, 1949-2553
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!