Evaluation of a hydrophilic interaction liquid chromatography design space for sugars and sugar alcohols

•A novel HILIC design space for analysis of sugars and sugar alcohols is described.•Aerosol detectors were used for the analysis of sugars and sugar alcohols.•A novel algorithm for analysis of design space data is presented.•Use of the algorithm for efficient method development is demonstrated.•Appl...

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Bibliographic Details
Published in:Journal of Chromatography A Vol. 1489; pp. 65 - 74
Main Authors: Hetrick, Evan M., Kramer, Timothy T., Risley, Donald S.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 17.03.2017
Subjects:
acetone
acetonitrile
aerosols
Algorithms
CAD
Carbohydrate
Carbohydrates - analysis
Carbohydrates - isolation & purification
Chromatography, High Pressure Liquid - methods
computer software
detection limit
detectors
ELSD
glucose
HILIC
hydrophilic interaction chromatography
Hydrophobic and Hydrophilic Interactions
light scattering
quantitative analysis
Solvents - chemistry
Sugar
Sugar alcohols
Sugar Alcohols - analysis
Sugar Alcohols - isolation & purification
trehalose
ELSD
Sugar alcohols
Carbohydrate
HILIC
Sugar
CAD
ISSN:0021-9673, 1873-3778, 1873-3778
Online Access:Get full text
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Summary:•A novel HILIC design space for analysis of sugars and sugar alcohols is described.•Aerosol detectors were used for the analysis of sugars and sugar alcohols.•A novel algorithm for analysis of design space data is presented.•Use of the algorithm for efficient method development is demonstrated.•Application for impurity control for pharmaceutical development is described. Based on a column-screening exercise, a column ranking system was developed for sample mixtures containing any combination of 26 sugar and sugar alcohol analytes using 16 polar stationary phases in the HILIC mode with acetonitrile/water or acetone/water mobile phases. Each analyte was evaluated on the HILIC columns with gradient elution and the subsequent chromatography data was compiled into a statistical software package where any subset of the analytes can be selected and the columns are then ranked by the greatest separation. Since these analytes lack chromophores, aerosol-based detectors, including an evaporative light scattering detector (ELSD) and a charged aerosol detector (CAD) were employed for qualitative and quantitative detection. Example qualitative applications are provided to illustrate the practicality and efficiency of this HILIC column ranking. Furthermore, the design-space approach was used as a starting point for a quantitative method for the trace analysis of glucose in trehalose samples in a complex matrix. Knowledge gained from evaluating the design-space led to rapid development of a capable method as demonstrated through validation of the following parameters: specificity, accuracy, precision, linearity, limit of quantitation, limit of detection, and range.
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ISSN:0021-9673
1873-3778
1873-3778
DOI:10.1016/j.chroma.2017.01.072