The CRISPR-Cas toolbox and gene editing technologies

The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher effic...

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Bibliographic Details
Published in:Molecular cell Vol. 82; no. 2; p. 333
Main Authors: Liu, Guanwen, Lin, Qiupeng, Jin, Shuai, Gao, Caixia
Format: Journal Article
Language:English
Published: United States 20.01.2022
Subjects:
Animals
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Associated Proteins - genetics
CRISPR-Associated Proteins - metabolism
CRISPR-Cas Systems
Diffusion of Innovation
Gene Editing
Humans
ISSN:1097-4164, 1097-4164
Online Access:Get more information
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Summary:The emergence of CRISPR-Cas systems has accelerated the development of gene editing technologies, which are widely used in the life sciences. To improve the performance of these systems, workers have engineered and developed a variety of CRISPR-Cas tools with a broader range of targets, higher efficiency and specificity, and greater precision. Moreover, CRISPR-Cas-related technologies have also been expanded beyond making cuts in DNA by introducing functional elements that permit precise gene modification, control gene expression, make epigenetic changes, and so on. In this review, we introduce and summarize the characteristics and applications of different types of CRISPR-Cas tools. We discuss certain limitations of current approaches and future prospects for optimizing CRISPR-Cas systems.
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ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2021.12.002