Toll like receptor-9 agonists stimulate prostate cancer invasion in vitro

BACKGROUND Toll‐like receptor 9 (TLR9) recognizes microbial DNA. In addition to immune cells, TLR9 expression has been detected in various cancer cells. We showed recently that TLR9 agonistic CpG‐oligonucleotides (CpG‐ODNs) induce matrix metalloproteinase‐13 (MMP‐13)‐mediated invasion in TLR9‐expres...

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Vydáno v:	The Prostate Ročník 67; číslo 7; s. 774 - 781
Hlavní autoři:	Ilvesaro, Joanna M., Merrell, Melinda A., Swain, Telisha Millender, Davidson, Jennifer, Zayzafoon, Majd, Harris, Kevin W., Selander, Katri S.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.05.2007 Wiley-Liss
Témata:	Adjuvants, Immunologic - pharmacology Antimalarials - pharmacology Biological and medical sciences Cell Line, Tumor Chloroquine - pharmacology DNA, Bacterial - pharmacology Estradiol - pharmacology Gene Expression Regulation, Leukemic - drug effects Gynecology. Andrology. Obstetrics Humans Infection - complications invasion Male Male genital diseases Matrix Metalloproteinase 13 - genetics Matrix Metalloproteinase 13 - physiology matrix metalloproteinase-13 Medical sciences Neoplasm Invasiveness Nephrology. Urinary tract diseases Oligodeoxyribonucleotides - pharmacology prostate cancer Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Toll-like receptor 9 Toll-Like Receptor 9 - agonists Toll-Like Receptor 9 - genetics Toll-Like Receptor 9 - physiology Tumors Tumors of the urinary system Urinary tract. Prostate gland Andrology Agonist Nephrology Urinary system disease Prostate disease Gynecology Malignant tumor Toll-like receptor 9 In vitro Collagenase 3 Invasion Toll like receptor 9 matrix metalloproteinase-13 Male genital diseases Prostate cancer
ISSN:	0270-4137, 1097-0045
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Popis
Shrnutí:	BACKGROUND Toll‐like receptor 9 (TLR9) recognizes microbial DNA. In addition to immune cells, TLR9 expression has been detected in various cancer cells. We showed recently that TLR9 agonistic CpG‐oligonucleotides (CpG‐ODNs) induce matrix metalloproteinase‐13 (MMP‐13)‐mediated invasion in TLR9‐expressing (TLR9+) breast cancer cells. We investigated here TLR9 expression and function in human prostate cancer (CaP) cells. METHODS TLR9 expression was detected with Western blotting and immunohistochemistry. Invasion was studied with Matrigel‐assays. MMP‐13 was assayed with ELISA. RESULTS Human CaP cell lines and clinical samples exhibit various levels of TLR9 expression. Treatment of TLR9+, but not TLR9− CaP cells with CpG‐ODNs or bacterial DNA increased their invasion, which was inhibited with chloroquine. CpG‐ODN‐treatment also increased MMP‐13 activity and neutralizing anti‐MMP‐13 antibody prevented CpG‐ODN‐induced invasion in TLR9+ CaP cells. Estradiol up‐regulated TLR9 expression in LnCaP cells. CONCLUSIONS TLR9‐mediated invasion may represent a novel mechanism through which infections promote prostate cancer. Prostate 67: 774–781, 2007. © 2007 Wiley‐Liss, Inc.
Bibliografie:	ArticleID:PROS20562 istex:97BE15C3CAAE90F9CCC7175ACC7239A5004403B4 ark:/67375/WNG-B53JQ5HQ-S ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0270-4137 1097-0045
DOI:	10.1002/pros.20562