Lineage tracing reveals clonal progenitors and long-term persistence of tumor-specific T cells during immune checkpoint blockade

Paired single-cell RNA and T cell receptor sequencing (scRNA/TCR-seq) has allowed for enhanced resolution of clonal T cell dynamics in cancer. Here, we report a scRNA/TCR-seq analysis of 187,650 T cells from 31 tissue regions, including tumor, adjacent normal tissues, and lymph nodes (LN), from thre...

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Veröffentlicht in:	Cancer cell Jg. 41; H. 4; S. 776
Hauptverfasser:	Pai, Joy A, Hellmann, Matthew D, Sauter, Jennifer L, Mattar, Marissa, Rizvi, Hira, Woo, Hyung Jun, Shah, Nisargbhai, Nguyen, Evelyn M, Uddin, Fathema Z, Quintanal-Villalonga, Alvaro, Chan, Joseph M, Manoj, Parvathy, Allaj, Viola, Baine, Marina K, Bhanot, Umesh K, Jain, Mala, Linkov, Irina, Meng, Fanli, Brown, David, Chaft, Jamie E, Plodkowski, Andrew J, Gigoux, Mathieu, Won, Helen H, Sen, Triparna, Wells, Daniel K, Donoghue, Mark T A, de Stanchina, Elisa, Wolchok, Jedd D, Loomis, Brian, Merghoub, Taha, Rudin, Charles M, Chow, Andrew, Satpathy, Ansuman T
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 10.04.2023
Schlagworte:	Carcinoma, Non-Small-Cell Lung - drug therapy CD8-Positive T-Lymphocytes Clone Cells Humans Immune Checkpoint Inhibitors - therapeutic use Lung Neoplasms - drug therapy Receptors, Antigen, T-Cell Tumor Microenvironment lung cancer exhausted T cells tumor-specific T cells TCF-1 progenitor exhausted T cells immune checkpoint blockade single-cell RNA/TCR sequencing
ISSN:	1878-3686, 1878-3686
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Zusammenfassung:	Paired single-cell RNA and T cell receptor sequencing (scRNA/TCR-seq) has allowed for enhanced resolution of clonal T cell dynamics in cancer. Here, we report a scRNA/TCR-seq analysis of 187,650 T cells from 31 tissue regions, including tumor, adjacent normal tissues, and lymph nodes (LN), from three patients with non-small cell lung cancer after immune checkpoint blockade (ICB). Regions with viable cancer cells are enriched for exhausted CD8 T cells, regulatory CD4 T cells (Treg), and follicular helper CD4 T cells (TFH). Tracking T cell clonotypes across tissues, combined with neoantigen specificity assays, reveals that TFH and tumor-specific exhausted CD8 T cells are clonally linked to TCF7 SELL progenitors in tumor draining LNs, and progressive exhaustion trajectories of CD8 T, Treg, and TFH cells with proximity to the tumor microenvironment. Finally, longitudinal tracking of tumor-specific CD8 and CD4 T cell clones reveals persistence in the peripheral blood for years after ICB therapy.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1878-3686 1878-3686
DOI:	10.1016/j.ccell.2023.03.009