Changes of faecal microbiota in patients with Crohn's disease treated with an elemental diet and total parenteral nutrition

Intestinal microbiota contributes to the pathogenesis of Crohn's disease. Elemental diet and total parenteral nutrition are effective therapies for Crohn's disease; however, changes of microbiota as a result of both treatments have not been fully elucidated. To elucidate changes of faecal...

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Vydáno v:Digestive and liver disease Ročník 44; číslo 9; s. 736 - 742
Hlavní autoři: Shiga, Hisashi, Kajiura, Takayuki, Shinozaki, Junko, Takagi, Sho, Kinouchi, Yoshitaka, Takahashi, Seiichi, Negoro, Kenichi, Endo, Katsuya, Kakuta, Yoichi, Suzuki, Manabu, Shimosegawa, Tooru
Médium: Journal Article
Jazyk:angličtina
Vydáno: Netherlands Elsevier Ltd 01.09.2012
Témata:
Adolescent
Adult
Aged
Bacteroides fragilis - genetics
Bifidobacterium - genetics
Case-Control Studies
Clostridium - genetics
Crohn Disease - diet therapy
Crohn Disease - microbiology
Crohn Disease - therapy
Crohn's disease
DNA, Bacterial - analysis
Elemental diet
Enterococcus - genetics
Feces - microbiology
Female
Food, Formulated
Gastroenterology and Hepatology
Humans
Intestinal microbiota
Male
Metagenome - genetics
Middle Aged
Parenteral Nutrition, Total
Polymorphism, Restriction Fragment Length
Real-Time Polymerase Chain Reaction
T-RFLP
Young Adult
Crohn's disease
Elemental diet
Intestinal microbiota
T-RFLP
ISSN:1590-8658, 1878-3562, 1878-3562
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Shrnutí:Intestinal microbiota contributes to the pathogenesis of Crohn's disease. Elemental diet and total parenteral nutrition are effective therapies for Crohn's disease; however, changes of microbiota as a result of both treatments have not been fully elucidated. To elucidate changes of faecal microbiota in Crohn's disease patients treated with elemental diet and total parenteral nutrition. Stool samples were collected from 33 active Crohn's disease patients and 17 healthy subjects, and recollected after elemental diet (8 patients) and total parenteral nutrition (9 patients). Terminal restriction fragment length polymorphism analysis of bacterial 16srDNA was performed to evaluate the whole microbiota. Specific quantitative PCR was then used to determine populations of predominant bacterial groups. In Crohn's disease patients, the number of terminal restriction fragments, which reflects bacterial species, was significantly lower. Populations of total bacteria and Bifidobacterium were significantly lower and the ratio of Enterococcus was higher. The number of terminal restriction fragments was significantly decreased after total parenteral nutrition, but not after elemental diet. Population of Bacteroides fragilis significantly decreased after elemental diet, while population of Enterococcus significantly increased after total parenteral nutrition. Faecal microbiota in Crohn's disease patients was markedly different from healthy subjects. Species diversity was reduced by total parenteral nutrition, but not by elemental diet.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1590-8658
1878-3562
1878-3562
DOI:10.1016/j.dld.2012.04.014