Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat

Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly...

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Vydáno v:	Molecular plant-microbe interactions Ročník 16; číslo 11; s. 955 - 961
Hlavní autoři:	Kemp, Gabré, Bergmann, Carl W., Clay, Ron, Van der Westhuizen, Amie J., Pretorius, Zacharias A.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	St Paul, MN APS Press 01.11.2003 American Phytopathological Society The American Phytopathological Society
Témata:	amino acid sequences antagonists & inhibitors Antifungal Agents Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Biological and medical sciences Bipolaris sorokiniana cell walls chemistry classification drug effects Enzyme Inhibitors Enzyme Inhibitors - isolation & purification Fundamental and applied biological sciences. Psychology fungi Fungi - classification Fungi - drug effects Immunoblotting isolation & purification leaves Luminescent Measurements Microbial Sensitivity Tests pathogens pharmacology physiology Phytopathology. Animal pests. Plant and forest protection Plant Proteins Plant Proteins - chemistry Plant Proteins - isolation & purification Plant Proteins - pharmacology polygalacturonase Polygalacturonase - antagonists & inhibitors Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization stems Triticum Triticum - physiology Triticum aestivum wheat Monocotyledones Microbiology Enzyme Polygalacturonase Protein Gramineae Glycosidases Angiospermae Hydrolases Isolation Spermatophyta O-Glycosidases Triticum aestivum
ISSN:	0894-0282, 1943-7706
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Popis
Shrnutí:	Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.
Bibliografie:	SourceType-Scholarly Journals-1 ObjectType-General Information-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	0894-0282 1943-7706
DOI:	10.1094/MPMI.2003.16.11.955