Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat

Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly...

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Bibliographic Details
Published in:Molecular plant-microbe interactions Vol. 16; no. 11; pp. 955 - 961
Main Authors: Kemp, Gabré, Bergmann, Carl W., Clay, Ron, Van der Westhuizen, Amie J., Pretorius, Zacharias A.
Format: Journal Article
Language:English
Published: St Paul, MN APS Press 01.11.2003
American Phytopathological Society
The American Phytopathological Society
Subjects:
amino acid sequences
antagonists & inhibitors
Antifungal Agents
Antifungal Agents - isolation & purification
Antifungal Agents - pharmacology
Biological and medical sciences
Bipolaris sorokiniana
cell walls
chemistry
classification
drug effects
Enzyme Inhibitors
Enzyme Inhibitors - isolation & purification
Fundamental and applied biological sciences. Psychology
fungi
Fungi - classification
Fungi - drug effects
Immunoblotting
isolation & purification
leaves
Luminescent Measurements
Microbial Sensitivity Tests
pathogens
pharmacology
physiology
Phytopathology. Animal pests. Plant and forest protection
Plant Proteins
Plant Proteins - chemistry
Plant Proteins - isolation & purification
Plant Proteins - pharmacology
polygalacturonase
Polygalacturonase - antagonists & inhibitors
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
stems
Triticum
Triticum - physiology
Triticum aestivum
wheat
Monocotyledones
Microbiology
Enzyme
Polygalacturonase
Protein
Gramineae
Glycosidases
Angiospermae
Hydrolases
Isolation
Spermatophyta
O-Glycosidases
Triticum aestivum
ISSN:0894-0282, 1943-7706
Online Access:Get full text
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Summary:Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.
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ISSN:0894-0282
1943-7706
DOI:10.1094/MPMI.2003.16.11.955