Increased frequency (12%) of circulating chronic lymphocytic leukemia-like B-cell clones in healthy subjects using a highly sensitive multicolor flow cytometry approach

Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry...

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Bibliographic Details
Published in:Blood Vol. 114; no. 1; p. 33
Main Authors: Nieto, Wendy G, Almeida, Julia, Romero, Alfonso, Teodosio, Cristina, López, Antonio, Henriques, Ana F, Sánchez, Maria Luz, Jara-Acevedo, María, Rasillo, Ana, González, Marcos, Fernández-Navarro, Paulino, Vega, Tomás, Orfao, Alberto
Format: Journal Article
Language:English
Published: United States 02.07.2009
Subjects:
Adult
Aged
Aged, 80 and over
B-Lymphocytes - immunology
B-Lymphocytes - pathology
Diagnosis, Differential
Disease Progression
Female
Flow Cytometry - methods
Flow Cytometry - statistics & numerical data
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Humans
Immunophenotyping
Leukemia, Lymphocytic, Chronic, B-Cell - blood
Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis
Leukemia, Lymphocytic, Chronic, B-Cell - genetics
Leukemia, Lymphocytic, Chronic, B-Cell - immunology
Lymphocyte Count
Lymphocytosis - blood
Lymphocytosis - diagnosis
Lymphocytosis - genetics
Lymphocytosis - immunology
Male
Middle Aged
Precancerous Conditions - blood
Precancerous Conditions - diagnosis
Precancerous Conditions - genetics
Precancerous Conditions - immunology
Sensitivity and Specificity
ISSN:1528-0020, 1528-0020
Online Access:Get more information
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Summary:Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 10(9)/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)-like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 10(6). We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 +/- 0.13 x 10(9) cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used.
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ISSN:1528-0020
1528-0020
DOI:10.1182/blood-2009-01-197368