Stem-Loop Structures within mRNA Coding Sequences Activate Translation Initiation and Mediate Control by Small Regulatory RNAs

Initiation is the rate-limiting step of translation, and in bacteria, mRNA secondary structure has been extensively reported as limiting the efficiency of translation by occluding the ribosome-binding site. In striking contrast with this inhibitory effect, we report here that stem-loop structures lo...

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Bibliographic Details
Published in:Molecular cell Vol. 68; no. 1; p. 158
Main Authors: Jagodnik, Jonathan, Chiaruttini, Claude, Guillier, Maude
Format: Journal Article
Language:English
Published: United States 05.10.2017
Subjects:
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Base Pairing
Base Sequence
Carrier Proteins - genetics
Carrier Proteins - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Expression Regulation, Bacterial
Inverted Repeat Sequences
Iron - metabolism
Nucleic Acid Conformation
Open Reading Frames
Peptide Chain Initiation, Translational
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Ribosomes - genetics
Ribosomes - metabolism
RNA, Bacterial - genetics
RNA, Bacterial - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Untranslated - genetics
RNA, Small Untranslated - metabolism
BAM complex
enterobactin
Hfq
regulatory mechanism
non-coding RNA
regulatory RNA
toeprint
translation-activating structure
starting block
siderophore
ISSN:1097-4164, 1097-4164
Online Access:Get more information
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Summary:Initiation is the rate-limiting step of translation, and in bacteria, mRNA secondary structure has been extensively reported as limiting the efficiency of translation by occluding the ribosome-binding site. In striking contrast with this inhibitory effect, we report here that stem-loop structures located within coding sequences instead activate translation initiation of the Escherichia coli fepA and bamA mRNAs involved in iron acquisition and outer membrane proteins assembly, respectively. Both structures promote ribosome binding in vitro, independently of their nucleotide sequence. Moreover, two small regulatory RNAs, OmrA and OmrB, base pair to and most likely disrupt the fepA stem-loop structure, thereby repressing FepA synthesis. By expanding our understanding of how mRNA cis-acting elements regulate translation, these data challenge the widespread view of mRNA secondary structures as translation inhibitors and show that translation-activating elements embedded in coding sequences can be targeted by small RNAs to inhibit gene expression.
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ISSN:1097-4164
1097-4164
DOI:10.1016/j.molcel.2017.08.015