Antibacterial activity and mechanism of slightly acidic electrolyzed water against Shewanella putrefaciens and Staphylococcus saprophytic

The purpose of this study was to investigate the antimicrobial effect and mechanism of slightly acidic electrolyzed water (SAEW) against Shewanella putrefaciens (S. putrefaciens) and Staphylococcus saprophytic (S. saprophyticus). The results showed that SAEW exhibited strong antimicrobial activity a...

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Bibliographic Details
Published in:Biochemical and biophysical research communications Vol. 592; pp. 44 - 50
Main Authors: Liu, Lin, Lan, Weiqing, Wang, Yanbo, Xie, Jing
Format: Journal Article
Language:English
Published: United States Elsevier Inc 12.02.2022
Subjects:
Anti-Bacterial Agents - pharmacology
Antibacterial mechanism
antibacterial properties
Antioxidants - pharmacology
Bacterial Proteins - metabolism
Cell Membrane - drug effects
Cell Membrane - metabolism
cell structures
chlorine
death
DNA
DNA, Bacterial - metabolism
Electrolysis
electrolyzed water
membrane permeability
Membrane Potentials - drug effects
Microbial Sensitivity Tests
Models, Biological
mortality
reactive oxygen species
Reactive Oxygen Species - metabolism
saprophytes
Shewanella putrefaciens
Shewanella putrefaciens - drug effects
Shewanella putrefaciens - ultrastructure
Slightly acidic electrolyzed water
Staphylococcus - drug effects
Staphylococcus - ultrastructure
Staphylococcus saprophytic
Water - pharmacology
Slightly acidic electrolyzed water
Shewanella putrefaciens
Staphylococcus saprophytic
Antibacterial mechanism
ISSN:0006-291X, 1090-2104, 1090-2104
Online Access:Get full text
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Summary:The purpose of this study was to investigate the antimicrobial effect and mechanism of slightly acidic electrolyzed water (SAEW) against Shewanella putrefaciens (S. putrefaciens) and Staphylococcus saprophytic (S. saprophyticus). The results showed that SAEW exhibited strong antimicrobial activity against tested bacteria, which was positively correlated to the available chlorine concentration (ACC) of SAEW. The mortality rate of S. putrefaciens and S. saprophyticus was up to 96% and 85%, respectively, when the ACC of SAEW was 60.0 mg/L. The results of scanning electron microscopy (SEM) showed that the cell morphology and structure were destroyed by SAEW. Besides, the results of confocal laser scanning microscopy (CLSM), leakage of DNA and protein provided evidence that SAEW induced membrane permeabilization in cells. Compared with the control, the intracellular reactive oxygen species (ROS) generated by SAEW was strengthened significantly with the increase of ACC, and the cells were injured to death accordingly. [Display omitted] •SAEW exhibited a strong bactericidal ability against fish spoilage bacteria.•SAEW treatment could cause damage to the cell structure.•The release of ROS induced the necrosis of cells after SAEW treatment.•Intracellular antioxidant enzyme activity decreased after SAEW treatment.
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ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2022.01.013