Triazole-based osmium() complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium( ii ) complexes [Os( N ^ N ) 3 ] 2+ ( N ^ N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole ( 1 2+ ); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triaz...

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Published in:Chemical science (Cambridge) Vol. 11; no. 33; pp. 8928 - 8935
Main Authors: Smitten, Kirsty L, Scattergood, Paul A, Kiker, Charlotte, Thomas, Jim A, Elliott, Paul I. P
Format: Journal Article
Language:English
Published: England Royal Society of Chemistry 07.08.2020
The Royal Society of Chemistry
Subjects:
Absorption spectra
Antiinfectives and antibacterials
Antimicrobial agents
Aqueous solutions
Charge transfer
Chemistry
Image resolution
Isomers
Luminescence
Near infrared radiation
Osmium
Toxicity
Triazoles
ISSN:2041-6520, 2041-6539
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Summary:Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium( ii ) complexes [Os( N ^ N ) 3 ] 2+ ( N ^ N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole ( 1 2+ ); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole ( 2 2+ ); 1-benzyl-4-(pyrazin-2-yl)-1,2,3-triazole ( 3 2+ )) were prepared and isolated as the chloride salts of their meridional and facial isomers. The complexes display prominent spin-forbidden ground state to triplet metal-to-ligand charge transfer ( 3 MLCT) state absorption bands enabling excitation as low as 600 nm for fac / mer - 3 2+ and observation of emission in aqueous solution in the deep-red/near-IR regions of the spectrum. Cellular uptake studies within MRSA cells show antimicrobial activity for 1 2+ and 2 2+ with greater toxicity for the meridional isomers in each case and mer - 1 2+ showing the greatest potency (32 μg mL −1 in defined minimal media). Super-resolution imaging experiments demonstrate binding of mer - and fac - 1 2+ to bacterial DNA with high Pearson's colocalisation coefficients (up to 0.95 using DAPI). Phototoxicity studies showed the complexes exhibited a higher antimicrobial activity upon irradiation with light. Cellular uptake, luminescence imaging and antimicrobial activity of facial and meridional isomers of Os( ii ) triazole-based complexes against methicillin-resistant S. aureus , MRSA.
Bibliography:10.1039/d0sc03563g
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ISSN:2041-6520
2041-6539
DOI:10.1039/d0sc03563g