Triazole-based osmium() complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging
Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium( ii ) complexes [Os( N ^ N ) 3 ] 2+ ( N ^ N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole ( 1 2+ ); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triaz...
Uložené v:
| Vydané v: | Chemical science (Cambridge) Ročník 11; číslo 33; s. 8928 - 8935 |
|---|---|
| Hlavní autori: | , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
England
Royal Society of Chemistry
07.08.2020
The Royal Society of Chemistry |
| Predmet: | |
| ISSN: | 2041-6520, 2041-6539 |
| On-line prístup: | Získať plný text |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Shrnutí: | Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant
S. aureus
(MRSA) bacteria are reported. The osmium(
ii
) complexes [Os(
N
^
N
)
3
]
2+
(
N
^
N
= 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole (
1
2+
); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole (
2
2+
); 1-benzyl-4-(pyrazin-2-yl)-1,2,3-triazole (
3
2+
)) were prepared and isolated as the chloride salts of their meridional and facial isomers. The complexes display prominent spin-forbidden ground state to triplet metal-to-ligand charge transfer (
3
MLCT) state absorption bands enabling excitation as low as 600 nm for
fac
/
mer
-
3
2+
and observation of emission in aqueous solution in the deep-red/near-IR regions of the spectrum. Cellular uptake studies within MRSA cells show antimicrobial activity for
1
2+
and
2
2+
with greater toxicity for the meridional isomers in each case and
mer
-
1
2+
showing the greatest potency (32 μg mL
−1
in defined minimal media). Super-resolution imaging experiments demonstrate binding of
mer
- and
fac
-
1
2+
to bacterial DNA with high Pearson's colocalisation coefficients (up to 0.95 using DAPI). Phototoxicity studies showed the complexes exhibited a higher antimicrobial activity upon irradiation with light.
Cellular uptake, luminescence imaging and antimicrobial activity of facial and meridional isomers of Os(
ii
) triazole-based complexes against methicillin-resistant
S. aureus
, MRSA. |
|---|---|
| Bibliografia: | 10.1039/d0sc03563g Electronic supplementary information (ESI) available. See DOI ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ISSN: | 2041-6520 2041-6539 |
| DOI: | 10.1039/d0sc03563g |