Geometric approach to segmentation and protein localization in cell culture assays

Cell-based fluorescence imaging assays are heterogeneous and require the collection of a large number of images for detailed quantitative analysis. Complexities arise as a result of variation in spatial nonuniformity, shape, overlapping compartments and scale (size). A new technique and methodology...

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Veröffentlicht in:	Journal of microscopy (Oxford) Jg. 225; H. 1; S. 22 - 30
Hauptverfasser:	RAMAN, S, MAXWELL, C.A, BARCELLOS-HOFF, M.H, PARVIN, B
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Oxford, UK Blackwell Publishing Ltd 2007
Schlagworte:	Algorithms Cell Culture Techniques Cell Line Centrosomal abnormality Female fluorescence microscopy Humans Image Processing, Computer-Assisted Microscopy, Fluorescence - methods Microscopy, Fluorescence - statistics & numerical data Models, Biological protein localization Proteins - metabolism Software Subcellular Fractions - metabolism
ISSN:	0022-2720, 1365-2818
Online-Zugang:	Volltext
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