Inhibition of human lymphokine-activated killer activity by smokeless tobacco (snuff) extract
Chronic snuff dipping has been associated with oral cancer in man and experimental animals. Here, the effects of a water-extract of snuff on the in-vitro development of human lymphokine-activated killer (LAK) activity were examined. The snuff extract inhibited both LAK cytotoxicity and DNA synthesis...
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| Vydáno v: | Archives of oral biology Ročník 33; číslo 5; s. 317 |
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| Hlavní autoři: | , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
England
1988
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| Témata: | |
| ISSN: | 0003-9969 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
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| Shrnutí: | Chronic snuff dipping has been associated with oral cancer in man and experimental animals. Here, the effects of a water-extract of snuff on the in-vitro development of human lymphokine-activated killer (LAK) activity were examined. The snuff extract inhibited both LAK cytotoxicity and DNA synthesis in a dose-dependent fashion at concentrations of 0.125 to 2.0 per cent; above 2.0 per cent, cell viability decreased significantly. In contrast, the snuff extract had no effect on natural killer-cell cytotoxicity when incubated with fresh peripheral blood lymphocytes in a standard 4 h assay, or on LAK cytotoxicity when incubated only during the final 4 h effector phase. Lymphocyte protein synthesis was generally unaffected by the addition of this extract. Thus, a water-soluble snuff extract appears to suppress LAK activity by inhibiting DNA synthesis. Altered LAK function in the oral mucosa might permit the development of snuff-associated carcinogenesis. |
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| Bibliografie: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/0003-9969(88)90064-7 |