The Rid family member RutC of Escherichia coli is a 3-aminoacrylate deaminase

The Rid protein family (PF14588, IPR006175) is divided into nine subfamilies, of which only the RidA subfamily has been characterized biochemically. RutC, the founding member of one subfamily, is encoded in the pyrimidine utilization (rut) operon that encodes a pathway that allows Escherichia coli t...

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Veröffentlicht in:The Journal of biological chemistry Jg. 296; S. 100651
Hauptverfasser: Buckner, Brandi A., Lato, Ashley M., Campagna, Shawn R., Downs, Diana M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 01.01.2021
American Society for Biochemistry and Molecular Biology
Schlagworte:
3-aminoacrylate
Acrylates - metabolism
Aminohydrolases - genetics
Aminohydrolases - metabolism
deaminase
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Nitrogen - metabolism
Oxidoreductases - genetics
Oxidoreductases - metabolism
pyridoxal phosphate
Pyridoxal Phosphate - metabolism
Rid superfamily
rut
Salmonella enterica - enzymology
Substrate Specificity
Uracil - metabolism
pyridoxal phosphate
DMSO
deaminase
CdsH
2-AA
3-AA
rut
3-aminoacrylate
HRMS
NCE
LOX
NCN
Rid superfamily
ESI
ISSN:0021-9258, 1083-351X, 1083-351X
Online-Zugang:Volltext
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Zusammenfassung:The Rid protein family (PF14588, IPR006175) is divided into nine subfamilies, of which only the RidA subfamily has been characterized biochemically. RutC, the founding member of one subfamily, is encoded in the pyrimidine utilization (rut) operon that encodes a pathway that allows Escherichia coli to use uracil as a sole nitrogen source. Results reported herein demonstrate that RutC has 3-aminoacrylate deaminase activity and facilitates one of the reactions previously presumed to occur spontaneously in vivo. RutC was active with several enamine–imine substrates, showing similarities and differences in substrate specificity with the canonical member of the Rid superfamily, Salmonella enterica RidA. Under standard laboratory conditions, a Rut pathway lacking RutC generates sufficient nitrogen from uracil for growth of E. coli. These results support a revised model of the Rut pathway and provide evidence that Rid proteins may modulate metabolic fitness, rather than catalyzing essential functions.
Bibliographie:ObjectType-Article-1
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content type line 23
ISSN:0021-9258
1083-351X
1083-351X
DOI:10.1016/j.jbc.2021.100651