MALDI-MS-based imaging of small molecules and proteins in tissues

The direct analysis of tissues using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) enables both endogenous and exogenous compounds present in tissues to be detected with molecular specificity while maintaining their spatial orientation. This unique combination, coupled wit...

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Bibliographic Details
Published in:Current opinion in chemical biology Vol. 11; no. 1; pp. 29 - 35
Main Authors: Reyzer, Michelle L, Caprioli, Richard M
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.02.2007
Subjects:
Animals
Biomarkers - analysis
Cerebellum - chemistry
Cerebellum - metabolism
Cerebellum - pathology
Humans
Mice
Neoplasms - chemistry
Neoplasms - metabolism
Neoplasms - pathology
Neurosciences - methods
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Proteins - analysis
Proteins - chemistry
Proteins - metabolism
Rats
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - trends
Technology, Pharmaceutical - methods
Tissue Distribution
Tissue Extracts - analysis
Tissue Extracts - chemistry
Tissue Extracts - metabolism
ISSN:1367-5931, 1879-0402
Online Access:Get full text
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Summary:The direct analysis of tissues using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) enables both endogenous and exogenous compounds present in tissues to be detected with molecular specificity while maintaining their spatial orientation. This unique combination, coupled with excellent sensitivity and rapid analysis time, presents many potential advantages to a wide range of applications in diverse biological fields. Recent advances have shown how the technique can be applied to cancer research, neuroscience and pharmaceutical development. Examples include the use of unique protein profiles to classify human tumor tissues and predict patient outcomes, the discovery of protein changes in mouse cerebellum as a function of development, and the two-dimensional visualization of the distribution of a drug and first-pass metabolites in rat whole-body sections.
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ISSN:1367-5931
1879-0402
DOI:10.1016/j.cbpa.2006.11.035