Increased circulating T helper 17 (TH17) cells and endometrial tissue IL-17-producing cells in patients with endometriosis compared with non-endometriotic subjects

Endometriosis, an inflammatory disease, is characterized by the aberrant presence of endometrial tissues at ectopic locations. Accumulating evidence suggests that inflammatory cells, such as interleukin-17 (IL-17)-producing cells, may be involved in the pathogenesis of endometriosis. This investigat...

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Published in:Reproductive biology Vol. 25; no. 2; p. 101019
Main Authors: Delbandi, Ali-Akbar, Mahmoudi, Mahmoud, Shervin, Adel, Farhangnia, Pooya, Mohammadi, Tahereh, Zarnani, Amir-Hassan
Format: Journal Article
Language:English
Published: Poland Elsevier B.V 01.06.2025
Subjects:
Adult
CD4-positive T-lymphocytes
Endometrial tissue
Endometriosis
Endometriosis - blood
Endometriosis - immunology
Endometriosis - metabolism
Endometriosis - pathology
endometrium
Endometrium - metabolism
Endometrium - pathology
Female
flow cytometry
Humans
IL-17
immunohistochemistry
interleukin-17
Interleukin-17 - metabolism
Leukocytes, Mononuclear - metabolism
pathogenesis
PBMCs
TH17 cells
Th17 Cells - metabolism
IL
TNF-α
IHC
PF
Treg
IL-17
Endometriosis
Endometrial tissue
PBMC
TH17 cells
PBMCs
T(H)17 cells
ISSN:1642-431X, 2300-732X, 2300-732X
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Summary:Endometriosis, an inflammatory disease, is characterized by the aberrant presence of endometrial tissues at ectopic locations. Accumulating evidence suggests that inflammatory cells, such as interleukin-17 (IL-17)-producing cells, may be involved in the pathogenesis of endometriosis. This investigation assessed the frequency of IL-17A (commonly known as IL-17)-producing cells in peripheral blood mononuclear cells (PBMCs), ectopic, and eutopic endometrial tissues in patients with endometriosis compared to non-endometriotic subjects. PBMCs, ectopic, and eutopic endometrial tissues were collected from 23 patients with endometriosis. PBMCs and endometrial tissues from 20 non-endometriotic women were used as the control group. The frequency of T helper 17 (TH17) lymphocytes in PBMCs was assessed using flow cytometry, and the expression level of IL-17 in eutopic and ectopic endometrial tissues was evaluated through immunohistochemistry. The percentage of TH17 and IL-17-producing lymphocytes was significantly higher in the PBMCs of patients with endometriosis compared to non-endometriotic subjects (P < 0.01 and P < 0.001, respectively). The expression of IL-17 protein in ectopic (P < 0.001) and eutopic (P < 0.05) endometrial tissues of patients with endometriosis increased compared to controls' endometrial tissue. Furthermore, the eutopic endometrium of patients with endometriosis showed a higher expression of IL-17 protein than the eutopic endometrial tissue of control subjects (P < 0.05). The findings suggest that the higher frequency of IL-17-producing cells in the PBMCs and endometrial tissues of patients with endometriosis contributes to the pathogenesis of endometriosis. •Women with endometriosis exhibit a significantly higher frequency of IL-17-producing cells in their PBMCs compared to control.•The expression of IL-17 protein is markedly higher in endometrial tissues of patients with endometriosis compared to control.•Elevated IL-17 levels in endometrial tissues and PBMCs may play a crucial role in the pathogenesis of endometriosis.
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ISSN:1642-431X
2300-732X
2300-732X
DOI:10.1016/j.repbio.2025.101019