Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1,2-epoxyoctane

Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was ext...

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Vydané v:	Biotechnology letters Ročník 25; číslo 20; s. 1775 - 1781
Hlavní autori:	Maritz, J., Krieg, H.M., Yeates, C.A., Botes, A.L., Breytenbach, J.C.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Dordrecht Springer 01.10.2003 Springer Nature B.V
Predmet:	1,2-epoxyoctane Alginates Basidiomycota Basidiomycota - growth & development Basidiomycota - metabolism Biological and medical sciences Biotechnology Calcium calcium alginate calcium chloride Cell Culture Techniques Cell Culture Techniques - methods Cells, Immobilized Cells, Immobilized - physiology Enzymatic activity Enzyme Activation enzyme activity Enzyme Stability Enzymes epoxide hydrolase Epoxide Hydrolases Epoxide Hydrolases - metabolism Epoxy Compounds Epoxy Compounds - metabolism Extracellular Matrix Extracellular Matrix - physiology Fundamental and applied biological sciences. Psychology Glucuronic Acid Glucuronic Acid - physiology growth & development Hexuronic Acids immobilized cells Kinetics metabolism methods Microspheres Octanes Octanes - metabolism physiology Rhodosporidium toruloides Stereoisomerism Yeasts Yeast Calcium Enzyme Immobilization Basidiomycetes calcium alginate Alginates Epoxide hydrolase Fungi Ether hydrolases immobilisation Hydrolases Kinetics (R,S)-1,2- epoxyoctane Thallophyta Rhodosporidium toruloides
ISSN:	0141-5492, 1573-6776
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Shrnutí:	Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilised biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M were utilised for bead preparation.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1026044113856