Calcium alginate entrapment of the yeast Rhodosporidium toruloides for the kinetic resolution of 1,2-epoxyoctane

Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was ext...

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Bibliographic Details
Published in:Biotechnology letters Vol. 25; no. 20; pp. 1775 - 1781
Main Authors: Maritz, J., Krieg, H.M., Yeates, C.A., Botes, A.L., Breytenbach, J.C.
Format: Journal Article
Language:English
Published: Dordrecht Springer 01.10.2003
Springer Nature B.V
Subjects:
1,2-epoxyoctane
Alginates
Basidiomycota
Basidiomycota - growth & development
Basidiomycota - metabolism
Biological and medical sciences
Biotechnology
Calcium
calcium alginate
calcium chloride
Cell Culture Techniques
Cell Culture Techniques - methods
Cells, Immobilized
Cells, Immobilized - physiology
Enzymatic activity
Enzyme Activation
enzyme activity
Enzyme Stability
Enzymes
epoxide hydrolase
Epoxide Hydrolases
Epoxide Hydrolases - metabolism
Epoxy Compounds
Epoxy Compounds - metabolism
Extracellular Matrix
Extracellular Matrix - physiology
Fundamental and applied biological sciences. Psychology
Glucuronic Acid
Glucuronic Acid - physiology
growth & development
Hexuronic Acids
immobilized cells
Kinetics
metabolism
methods
Microspheres
Octanes
Octanes - metabolism
physiology
Rhodosporidium toruloides
Stereoisomerism
Yeasts
Yeast
Calcium
Enzyme
Immobilization
Basidiomycetes
calcium alginate
Alginates
Epoxide hydrolase
Fungi
Ether hydrolases
immobilisation
Hydrolases
Kinetics
(R,S)-1,2- epoxyoctane
Thallophyta
Rhodosporidium toruloides
ISSN:0141-5492, 1573-6776
Online Access:Get full text
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Summary:Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilised biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M were utilised for bead preparation.
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ISSN:0141-5492
1573-6776
DOI:10.1023/A:1026044113856