An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination

In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein con...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry Vol. 480; pp. 28 - 30
Main Authors: Grintzalis, Konstantinos, Georgiou, Christos D., Schneider, Yves-Jacques
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.07.2015
Subjects:
bioassays
Bradford
Coomassie Brilliant Blue G (CBB)
dyes
Protein quantification
proteins
Proteins - analysis
protocols
rapid methods
Rosaniline Dyes - chemistry
Coomassie Brilliant Blue G (CBB)
Bradford
Protein quantification
ISSN:0003-2697, 1096-0309, 1096-0309
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
1096-0309
DOI:10.1016/j.ab.2015.03.024