An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination

In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein con...

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Veröffentlicht in:Analytical biochemistry Jg. 480; S. 28 - 30
Hauptverfasser: Grintzalis, Konstantinos, Georgiou, Christos D., Schneider, Yves-Jacques
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Elsevier Inc 01.07.2015
Schlagworte:
bioassays
Bradford
Coomassie Brilliant Blue G (CBB)
dyes
Protein quantification
proteins
Proteins - analysis
protocols
rapid methods
Rosaniline Dyes - chemistry
Coomassie Brilliant Blue G (CBB)
Bradford
Protein quantification
ISSN:0003-2697, 1096-0309, 1096-0309
Online-Zugang:Volltext
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Zusammenfassung:In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
1096-0309
DOI:10.1016/j.ab.2015.03.024