Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents

Bacteria of the genus Staphylococcus are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use of bacteriophages...

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Veröffentlicht in:Viruses Jg. 16; H. 3; S. 385
Hauptverfasser: Golosova, Natalia N., Matveev, Andrey L., Tikunova, Nina V., Khlusevich, Yana A., Kozlova, Yulia N., Morozova, Vera V., Babkin, Igor V., Ushakova, Tatiana A., Zhirakovskaya, Elena V., Panina, Elizaveta A., Ryabchikova, Elena I., Tikunov, Artem Y.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 29.02.2024
Schlagworte:
Adsorption
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibiotics
antimicrobial agent
Bacteria
Bacterial infections
bacteriophage
bacteriophages
Bacteriophages - genetics
biofilm
Biofilms
catalytic activity
Cell walls
Coagulase
coagulase negative staphylococci
Drug resistance
endolysin
Endopeptidases
Enzymes
Escherichia coli
genome
Genomes
Gram-positive bacteria
Humans
Lytic enzymes
medicine
Microscopy
Orthopedics
Pathogens
Patients
Peptidoglycans
Phages
plankton
Planktonic cells
Polyethylene glycol
Staphylococcal Infections - microbiology
Staphylococcus
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus haemolyticus
Staphylococcus infections
Staphylococcus warneri
Strains (organisms)
therapeutics
United States > US
Germany
antimicrobial agent
Staphylococcus epidermidis
endolysin
bacteriophage
Staphylococcus aureus
biofilm
ISSN:1999-4915, 1999-4915
Online-Zugang:Volltext
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Zusammenfassung:Bacteria of the genus Staphylococcus are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use of bacteriophages specific to these bacteria or the lytic enzymes of such bacteriophages, which are capable of hydrolyzing the cell walls of these bacteria. In this study, a new bacteriophage vB_SepP_134 (St 134) specific to Staphylococcus epidermidis was described. This podophage, with a genome of 18,275 bp, belongs to the Andhravirus genus. St 134 was able to infect various strains of 12 of the 21 tested coagulase-negative Staphylococcus species and one clinical strain from the Staphylococcus aureus complex. The genes encoding endolysin (LysSte134_1) and tail tip lysin (LysSte134_2) were identified in the St 134 genome. Both enzymes were cloned and produced in Escherichia coli cells. The endolysin LysSte134_1 demonstrated catalytic activity against peptidoglycans isolated from S. aureus, S. epidermidis, Staphylococcus haemolyticus, and Staphylococcus warneri. LysSte134_1 was active against S. aureus and S. epidermidis planktonic cells and destroyed the biofilms formed by clinical strains of S. aureus and S. epidermidis.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1999-4915
1999-4915
DOI:10.3390/v16030385