StM171, a Stenotrophomonas maltophilia Bacteriophage That Affects Sensitivity to Antibiotics in Host Bacteria and Their Biofilm Formation

Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacte...

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Bibliographic Details
Published in:Viruses Vol. 15; no. 12; p. 2455
Main Authors: Jdeed, Ghadeer, Morozova, Vera, Kozlova, Yuliya, Tikunov, Artem, Ushakova, Tatyana, Bardasheva, Alevtina, Manakhov, Andrey, Mitina, Maria, Zhirakovskaya, Elena, Tikunova, Nina
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 18.12.2023
Subjects:
Antibiotic resistance
Antibiotics
Bacteria
Bacterial infections
bacteriophage
bacteriophages
biofilm
biofilm formation
Biofilms
Burst size
Caudoviricetes
Cell culture
cephalosporins
comparative genomics
DNA
Drug resistance
Erythromycin
genome
Genomic analysis
genomics
hospitals
Host range
Immunocompromised hosts
Infections
Medical wastes
Microscopy
mortality
Mutants
Patients
Penicillin
Phages
Respiratory tract infection
Stenotrophomonas maltophilia
Strains (organisms)
wastewater
Xanthomonas
United States > US
Russia
Stenotrophomonas maltophilia
Caudoviricetes
biofilm formation
antibiotic resistance
bacteriophage
comparative genomics
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Summary:Stenotrophomonas maltophilia mainly causes respiratory infections that are associated with a high mortality rate among immunocompromised patients. S. maltophilia exhibits a high level of antibiotic resistance and can form biofilms, which complicates the treatment of patients infected with this bacterium. Phages combined with antibiotics could be a promising treatment option. Currently, ~60 S. maltophilia phages are known, and their effects on biofilm formation and antibiotic sensitivity require further examination. Bacteriophage StM171, which was isolated from hospital wastewater, showed a medium host range, low burst size, and low lytic activity. StM171 has a 44kbp dsDNA genome that encodes 59 open-reading frames. A comparative genomic analysis indicated that StM171, along with the Stenotrophomonas phage Suso (MZ326866) and Xanthomonas phage HXX_Dennis (ON711490), are members of a new putative Nordvirus genus. S. maltophilia strains that developed resistance to StM171 (bacterial-insensitive mutants) showed a changed sensitivity to antibiotics compared to the originally susceptible strains. Some bacterial-insensitive mutants restored sensitivity to cephalosporin and penicillin-like antibiotics and became resistant to erythromycin. StM171 shows strain- and antibiotic-dependent effects on the biofilm formation of S. maltophilia strains.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v15122455