StenM_174: A Novel Podophage That Infects a Wide Range of Stenotrophomonas spp. and Suggests a New Subfamily in the Family Autographiviridae

Stenotrophomonas maltophilia was discovered as a soil bacterium associated with the rhizosphere. Later, S. maltophilia was found to be a multidrug-resistant hospital-associated pathogen. Lytic bacteriophages are prospective antimicrobials; therefore, there is a need for the isolation and characteriz...

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Vydáno v:Viruses Ročník 16; číslo 1; s. 18
Hlavní autoři: Morozova, Vera V., Yakubovskij, Vyacheslav I., Baykov, Ivan K., Kozlova, Yuliya N., Tikunov, Artem Yu, Babkin, Igor V., Bardasheva, Alevtina V., Zhirakovskaya, Elena V., Tikunova, Nina V.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 21.12.2023
Témata:
anti-infective agents
Antibiotics
Antimicrobial agents
Autographiviridae
bacteriophages
Comparative analysis
comparative genomics
data analysis
farms
genome
Genomes
Hospitals
Infections
Morphology
morphs
Multidrug resistance
multiple drug resistance
new subfamily
nucleotide sequences
Pathogens
Phages
Podoviridae
poultry
Poultry farming
receptor-binding protein
Rhizosphere
soil bacteria
Soil microorganisms
Stenotrophomonas
Stenotrophomonas maltophilia
tail spike
virulent phage
United States > US
Stenotrophomonas maltophilia
receptor-binding protein
tail spike
virulent phage
AlphaFold
structural modeling
comparative genomics
Autographiviridae
ISSN:1999-4915, 1999-4915
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Shrnutí:Stenotrophomonas maltophilia was discovered as a soil bacterium associated with the rhizosphere. Later, S. maltophilia was found to be a multidrug-resistant hospital-associated pathogen. Lytic bacteriophages are prospective antimicrobials; therefore, there is a need for the isolation and characterization of new Stenotrophomonas phages. The phage StenM_174 was isolated from litter at a poultry farm using a clinical strain of S. maltophilia as the host. StenM_174 reproduced in a wide range of clinical and environmental strains of Stenotrophomonas, mainly S. maltophilia, and it had a podovirus morphotype. The length of the genomic sequence of StenM_174 was 42,956 bp, and it contained 52 putative genes. All genes were unidirectional, and 31 of them encoded proteins with predicted functions, while the remaining 21 were identified as hypothetical ones. Two tail spike proteins of StenM_174 were predicted using AlphaFold2 structural modeling. A comparative analysis of the genome shows that the Stenotrophomonas phage StenM_174, along with the phages Ponderosa, Pepon, Ptah, and TS-10, can be members of the new putative genus Ponderosavirus in the Autographiviridae family. In addition, the analyzed data suggest a new subfamily within this family.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1999-4915
1999-4915
DOI:10.3390/v16010018