Long non-coding RNA TUG1 mediates 5-fluorouracil resistance by acting as a ceRNA of miR-197-3p in colorectal cancer

One major reason for the failure of advanced colorectal cancer (CRC) treatment is the occurrence of chemoresistance to fluoropyrimidine (Fu)-based chemotherapy. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play a critical role in cancerous processes as either oncogenes or tumo...

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Veröffentlicht in:Journal of Cancer Jg. 10; H. 19; S. 4603 - 4613
Hauptverfasser: Wang, Meng, Hu, Hanqing, Wang, Yuliuming, Huang, Quanlong, Huang, Rui, Chen, Yinggang, Ma, Tianyi, Qiao, Tianyu, Zhang, Qian, Wu, Hongyu, Chen, Qingmin, Han, Dong, Wang, Guiyu, Wang, Xishan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Wyoming Ivyspring International Publisher Pty Ltd 01.01.2019
Ivyspring International Publisher
Schlagworte:
Cancer therapies
Chemotherapy
Colorectal cancer
Flow cytometry
Gene expression
Medical research
Reagents
Research Paper
Surgery
United States > US
China
ISSN:1837-9664, 1837-9664
Online-Zugang:Volltext
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Zusammenfassung:One major reason for the failure of advanced colorectal cancer (CRC) treatment is the occurrence of chemoresistance to fluoropyrimidine (Fu)-based chemotherapy. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play a critical role in cancerous processes as either oncogenes or tumor suppressor genes. Here, we observed lncRNA TUG1 was associated to the 5-Fu resistance in colorectal cancer. Firstly, quantitative analysis indicated that TUG1 was significantly increased in recurrence CRC patient samples. Kaplan-Meier survival analysis indicated that high TUG1 expression in CRC tissues was significantly associated with a higher rate of disease progression. TUG1 knockdown re-sensitized the 5-Fu resistance in colorectal cancer cells, which were 5-Fu-resistant colorectal cell line. Furthermore, bioinformatics analysis showed that miR-197-3p could directly bind to TUG1 suggesting TUG1 might work as a ceRNA to sponge miR-197-3p. Extensively, our study also showed that TYMS was the direct target of miR-197-3p in CRC cells. Taken together, our study suggests that TUG1 mediates 5-Fu resistance in CRC via miR-197-3p/TYMS axis.
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These authors contributed equally to the work and should be regarded as joint first authors.
Competing Interests: The authors have declared that no competing interest exists.
ISSN:1837-9664
1837-9664
DOI:10.7150/jca.32065