beta-1 Integrins mediate tumour cell adhesion to quiescent endothelial cells in vitro

Metastatic spread of some solid tumours is thought to depend upon the adhesion of tumour cells to the vascular endothelium followed by extravasation into surrounding tissues. We investigated the role of beta 1 integrins in the adhesion of the breast adenocarcinoma cell line MDA-MB-231 and the melano...

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Bibliographic Details
Published in:British journal of cancer Vol. 74; no. 11; p. 1762
Main Authors: Price, E A, Coombe, D R, Murray, J C
Format: Journal Article
Language:English
Published: England 01.12.1996
Subjects:
Breast Neoplasms - metabolism
Breast Neoplasms - physiopathology
Cell Adhesion - physiology
Endothelium, Vascular - metabolism
Endothelium, Vascular - physiopathology
Female
Humans
Integrin beta1 - physiology
Intercellular Junctions - metabolism
Intercellular Junctions - physiology
Melanoma - metabolism
Melanoma - physiopathology
Neoplasms - physiopathology
Tumor Cells, Cultured
Umbilical Veins
Vascular Cell Adhesion Molecule-1 - physiology
ISSN:0007-0920
Online Access:Get more information
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Summary:Metastatic spread of some solid tumours is thought to depend upon the adhesion of tumour cells to the vascular endothelium followed by extravasation into surrounding tissues. We investigated the role of beta 1 integrins in the adhesion of the breast adenocarcinoma cell line MDA-MB-231 and the melanoma cell line RPMI-7951 to quiescent human umbilical vein endothelial cells (HUVEC) in vitro. In the course of adhesion assays, tumour cells were observed to adhere to quiescent HUVEC monolayers, particularly at endothelial cell-cell junctions. Immunohistochemistry revealed concentration of beta 1 integrin expression at these sites. Adhesion was reduced by pretreatment of either tumour cells or HUVEC with antibodies against beta 1 integrins. Simultaneous treatment of HUVECs and tumour cells with these antibodies produced an additive blocking effect, consistent with a heterotypic adhesion mechanism. Our data suggest that tumour cell and endothelial beta 1 integrins may play a crucial role in the arrest and migration of tumour cells through the vascular endothelium in the absence of endothelial 'activation'.
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ISSN:0007-0920
DOI:10.1038/bjc.1996.627