Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats

As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon d...

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Published in:Frontiers in pharmacology Vol. 14; p. 1118550
Main Authors: Zhang, Meiling, Cheng, Jinjun, Luo, Juan, Li, Changxiang, Hou, Tingting, Zhao, Yan, Wang, Yaoxian, Qu, Huihua, Kong, Hui
Format: Journal Article
Language:English
Published: Frontiers Media S.A 10.08.2023
Subjects:
active ingredients
anti-inflammation
cooling blood and hemostatic effects
hemostasis
Pharmacology
Scutellariae Radix Carbonisata-derived carbon dots (SRC-CDs)
ISSN:1663-9812, 1663-9812
Online Access:Get full text
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Summary:As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines.
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Edited by: Ying-Yong Zhao, Northwest University, China
Reviewed by: Kunming Qin, Jiangsu Ocean Universiity, China
These authors have contributed equally to this work
Liying Huang, Fuajin Medical University, China
ISSN:1663-9812
1663-9812
DOI:10.3389/fphar.2023.1118550