Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors

We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essential...

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Veröffentlicht in:	Nature methods Jg. 5; H. 3; S. 253 - 260
Hauptverfasser:	Romanov, Sergei, Medvedev, Alexander, Gambarian, Maria, Poltoratskaya, Natalia, Moeser, Matt, Medvedeva, Liubov, Gambarian, Mikhail, Diatchenko, Luda, Makarov, Sergei
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.03.2008 Nature Publishing Group
Schlagworte:	Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedical research Carbazoles - pharmacology Cell Line, Tumor Cellular biology Cellular signal transduction Colforsin - pharmacology DNA binding proteins Gene Expression Profiling - methods Gene Library Genes, Reporter - physiology Genetic aspects Genetic regulation Homogeneity Humans Indoles - pharmacology Life Sciences Oligonucleotide Array Sequence Analysis - methods Physiological aspects Polymerase Chain Reaction Proteomics Rats Receptors, Aryl Hydrocarbon - drug effects Reproducibility of Results Research methodology Signal transduction Transcription Factors - genetics Transfection - methods Transforming Growth Factor beta - pharmacology United States
ISSN:	1548-7091, 1548-7105, 1548-7105
Online-Zugang:	Volltext
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