Methodological Challenges in Protein Microarray and Immunohistochemistry for the Discovery of Novel Autoantibodies in Paediatric Acute Disseminated Encephalomyelitis

Acute disseminated encephalomyelitis (ADEM) is a rare autoimmune-mediated demyelinating disease affecting mainly children and young adults. Differentiation to multiple sclerosis is not always possible, due to overlapping clinical symptoms and recurrent and multiphasic forms. Until now, immunoglobuli...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences Vol. 18; no. 3; p. 679
Main Authors: Peschl, Patrick, Ramberger, Melanie, Höftberger, Romana, Jöhrer, Karin, Baumann, Matthias, Rostásy, Kevin, Reindl, Markus
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 22.03.2017
MDPI
Subjects:
Animals
Antigens
Autoantibodies - immunology
Autoantigens - immunology
Biomarkers
Brain research
Child
Cluster Analysis
Cross Reactions - immunology
Disease
Encephalomyelitis, Acute Disseminated - diagnosis
Encephalomyelitis, Acute Disseminated - immunology
Female
Glycoproteins
Humans
Immunoglobulins
Immunohistochemistry
Male
Medical research
Membrane Proteins - immunology
Multiple sclerosis
Myelin-Oligodendrocyte Glycoprotein - immunology
Nerve Tissue Proteins - immunology
Neurology
Patients
Peptides
Potassium
Protein Array Analysis
Proteins
Proteins - immunology
Proteome
Proteomics - methods
Rats
Studies
Austria
acute disseminated encephalomyelitis
autoantibody
immunohistochemistry
autoantigen
protein microarray
paediatric
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute disseminated encephalomyelitis (ADEM) is a rare autoimmune-mediated demyelinating disease affecting mainly children and young adults. Differentiation to multiple sclerosis is not always possible, due to overlapping clinical symptoms and recurrent and multiphasic forms. Until now, immunoglobulins reactive to myelin oligodendrocyte glycoprotein (MOG antibodies) have been found in a subset of patients with ADEM. However, there are still patients lacking autoantibodies, necessitating the identification of new autoantibodies as biomarkers in those patients. Therefore, we aimed to identify novel autoantibody targets in ADEM patients. Sixteen ADEM patients (11 seronegative, 5 seropositive for MOG antibodies) were analysed for potential new biomarkers, using a protein microarray and immunohistochemistry on rat brain tissue to identify antibodies against intracellular and surface neuronal and glial antigens. Nine candidate antigens were identified in the protein microarray analysis in at least two patients per group. Immunohistochemistry on rat brain tissue did not reveal new target antigens. Although no new autoantibody targets could be found here, future studies should aim to identify new biomarkers for therapeutic and prognostic purposes. The microarray analysis and immunohistochemistry methods used here have several limitations, which should be considered in future searches for biomarkers.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms18030679