Methodological Challenges in Protein Microarray and Immunohistochemistry for the Discovery of Novel Autoantibodies in Paediatric Acute Disseminated Encephalomyelitis

Acute disseminated encephalomyelitis (ADEM) is a rare autoimmune-mediated demyelinating disease affecting mainly children and young adults. Differentiation to multiple sclerosis is not always possible, due to overlapping clinical symptoms and recurrent and multiphasic forms. Until now, immunoglobuli...

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Vydáno v:	International journal of molecular sciences Ročník 18; číslo 3; s. 679
Hlavní autoři:	Peschl, Patrick, Ramberger, Melanie, Höftberger, Romana, Jöhrer, Karin, Baumann, Matthias, Rostásy, Kevin, Reindl, Markus
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland MDPI AG 22.03.2017 MDPI
Témata:	Animals Antigens Autoantibodies - immunology Autoantigens - immunology Biomarkers Brain research Child Cluster Analysis Cross Reactions - immunology Disease Encephalomyelitis, Acute Disseminated - diagnosis Encephalomyelitis, Acute Disseminated - immunology Female Glycoproteins Humans Immunoglobulins Immunohistochemistry Male Medical research Membrane Proteins - immunology Multiple sclerosis Myelin-Oligodendrocyte Glycoprotein - immunology Nerve Tissue Proteins - immunology Neurology Patients Peptides Potassium Protein Array Analysis Proteins Proteins - immunology Proteome Proteomics - methods Rats Studies Austria acute disseminated encephalomyelitis autoantibody immunohistochemistry autoantigen protein microarray paediatric
ISSN:	1422-0067, 1661-6596, 1422-0067
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Popis
Shrnutí:	Acute disseminated encephalomyelitis (ADEM) is a rare autoimmune-mediated demyelinating disease affecting mainly children and young adults. Differentiation to multiple sclerosis is not always possible, due to overlapping clinical symptoms and recurrent and multiphasic forms. Until now, immunoglobulins reactive to myelin oligodendrocyte glycoprotein (MOG antibodies) have been found in a subset of patients with ADEM. However, there are still patients lacking autoantibodies, necessitating the identification of new autoantibodies as biomarkers in those patients. Therefore, we aimed to identify novel autoantibody targets in ADEM patients. Sixteen ADEM patients (11 seronegative, 5 seropositive for MOG antibodies) were analysed for potential new biomarkers, using a protein microarray and immunohistochemistry on rat brain tissue to identify antibodies against intracellular and surface neuronal and glial antigens. Nine candidate antigens were identified in the protein microarray analysis in at least two patients per group. Immunohistochemistry on rat brain tissue did not reveal new target antigens. Although no new autoantibody targets could be found here, future studies should aim to identify new biomarkers for therapeutic and prognostic purposes. The microarray analysis and immunohistochemistry methods used here have several limitations, which should be considered in future searches for biomarkers.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work.
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms18030679