Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance

We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an...

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Veröffentlicht in:Journal of clinical microbiology Jg. 51; H. 7; S. 2453
Hauptverfasser: Mancini, Nicasio, De Carolis, Elena, Infurnari, Laura, Vella, Antonietta, Clementi, Nicola, Vaccaro, Luisa, Ruggeri, Alberto, Posteraro, Brunella, Burioni, Roberto, Clementi, Massimo, Sanguinetti, Maurizio
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.07.2013
Schlagworte:
Diagnostic Errors - statistics & numerical data
Humans
Microbiological Techniques - methods
Mycological Typing Techniques - methods
Mycology - methods
Mycoses - diagnosis
Mycoses - microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
Yeasts - chemistry
Yeasts - classification
Yeasts - isolation & purification
Yeasts - metabolism
ISSN:1098-660X, 1098-660X
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Zusammenfassung:We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).
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ISSN:1098-660X
1098-660X
DOI:10.1128/JCM.00841-13